基于SLAF-seq技术分析甜、糯玉米种质遗传多样性
利用简化基因组测序技术SLAF-seq,对国内外引进选育的81份鲜食甜、糯玉米自交系进行遗传多样性分析。结果表明,共获得 803 058个SLAF 标签, 其中多态性 SLAF 标签 373 764个。通过序列分析,获得 169 128个有效单核苷酸(SNP)多态标记, 利用这些SNP标记分析和构建了81份鲜食甜、糯玉米资源的群体结构和系统发生树,并将其分为2个群。研究结果表明,简化基因组测序技术SLAF-seq能高效、低廉地开发出大量SNP标记,是作物种质资源群体遗传分析的有效工具；该研究结果为甜玉米不同基因的聚合、温-热种质杂交育种、鲜食甜、糯玉米亚种间杂交育种提供依据,也有利于鲜食甜、糯玉米自交系的高效利用、品种选育及杂种优势群的建立。
英文摘要:
High-throughput specific locus amplified fragment sequencing (SLAF-seq) technology was employed to identify the genetic diversity of 81 inbred lines include sweet and wax corn. The results indicated that,the total of 803 058 SLAF tags and 373 764 polymorphic SLAF tags were obtained. A total of 169 128 single nucleotide polymorphisms (SNPs) were obtained through sequences analysis. Population structure and phylogenetic relationship of 81 germplasms of fresh sweet and wax corn were analyzed using the SNP dataset, the population was significantly divided into two different groups. It suggests that SLAF-seq can be used to develop large-scale SNPs for population genetic analysis, effectively and economically. Our results can provide molecular basis for gene pyramiding of sweet corn, hybrid breeding of temperate-tropical mode and sweet-wax type. It is beneficial for promoting effective utilization of sweet corn inbred lines, breeding new cultivars and building of heterotic groups.
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