Plant Physiol:华南农大陆旺金研究组发表香蕉相关论文
近日，国际植物学顶级期刊《plant Physiology》杂志在线发表华南农业大学“亚热带农业生物资源保护与利用”国家重点实验室及园艺学院陆旺金教授为通讯作者的研究论文“Banana Transcription Factor MaERF11 Recruits Histone Deacetylase MaHDA1 and Represses the expression of MaACO1 and Expansins during Fruit Ripening”。该论文第一作者为陆旺金教授指导的博士生韩延超，共同第一作者为园艺学院青年教师邝健飞副研究员。
香蕉是呼吸跃变型果实，成熟启动后果实迅速软化，给香蕉采后保鲜物流带来较大困难。针对这个限制香蕉产业发展的问题，课题组开展了香蕉果实成熟转录调控研究，发现1个MaERF11转录因子与果实成熟可能密切相关，但MaERF11是如何调控果实成熟目前尚不清楚。该论文中，采用生物化学、表观遗传学和分子生物学等方法，进一步证实MaERF11与组蛋白去乙酰化酶MaHDA1相互作用，并共同调控乙烯合成和细胞壁降解等成熟相关基因的表达。MaERF11招募MaHDA1蛋白到成熟相关基因启动子的GCC-box区域，并介由MaHDA1的组蛋白去乙酰化酶活性，抑制成熟相关基因的转录表达，从而调控香蕉果实的成熟。文章的科学意义在于揭示了组蛋白去乙酰化参与调控果实成熟，这一研究成果不仅丰富了果实成熟的转录调控机制，也为果实保鲜技术研发提供了理论依据。
原文链接：
Banana Transcription Factor MaERF11 Recruits Histone DeAcetylase MaHDA1 and Represses the Expression of MaACO1 and Expansins during Fruit Ripening
原文摘要：
Phytohormone ethylene controls diverse developmental and physiological processes such as fruit ripening via modulation of ethylene signaling pathway. Our previous study identified that ETHYLENE RESPONSE FACTOR11 (MaERF11), a transcription factor in the ethylene signaling pathway, negatively regulates the ripening of banana, but the mechanism for the MaERF11-mediated transcriptional regulation remains largely unknown. Here we showed that MaERF11 has intrinsic transcriptional repression activity in planta. Electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) analyses demonstrated that MaERF11 binds to promoters of three ripening-related Expansin genes, MaEXP2, MaEXP7 and MaEXP8, as well as an ethylene biosynthetic gene MaACO1, via the GCC-box motif. Furthermore, expression patterns of MaACO1, MaEXP2, MaEXP7 and MaEXP8 genes are correlated with the changes of histone H3 and H4 acetylation level during fruit ripening. Moreover, we found that MaERF11 physically interacts with a histone deacetylase, MaHDA1, which has histone deacetylase activity, and the interaction significantly strengthened the MaERF11-mediated transcriptional repression of MaACO1 and Expansins. Taken together, these findings suggest that MaERF11 may recruit MaHDA1 to its target genes and repress their expression via histone deacetylation.
作者：陆旺金
