Front Plant SCI:中科院武汉植物园王瑛研究组和首尔大学研究学者发

2016年3月16日，《Frontiers in plant science》期刊杂志在线发表中国科学院武汉植物园王瑛研究员与韩国首尔大学教授Tae-Jin Yang合作发表的一篇研究论文，论文首次对我国 4 种淫羊藿属植物叶绿体基因组进行了全测序。研究结果对于淫羊藿属植物的系统演化分析、品种鉴定具有重要意义。张燕君副研究员为论文第一作者，Tae-Jin Yang教授和王瑛研究员为论文共同通讯作者。

淫羊藿为小檗科淫羊藿属植物，是我国常用大宗中药材，具有较多的药用价值和广阔的开发前景。当前淫羊藿药材主要依靠野生资源，存在基原植物混乱、质量参差不齐等问题。叶绿体基因组信息可以用于植物的系统分类、品种鉴定，但目前还没有对我国淫羊藿属植物开展过叶绿体基因组的研究。

研究人员首次对我国 4 种淫羊藿属植物叶绿体基因组进行了全测序。结合前期报道的韩国 1 个淫羊藿物种的叶绿体基因组序列，对淫羊藿属叶绿体基因组进行了深入的分析。研究结果表明不同淫羊藿物种间存在反向重复区(IR)收缩和扩张的差异;基因trnQ-UUG在大单拷贝区(LSC)发生了复制事件，这在其他基部双子叶植物中还未见报道。同时，该研究还筛选了一批淫羊藿叶绿体基因组的多态性dna片段、SSR位点、REPuter位点，可用于该属植物的系统分类、品种鉴定、居群遗传学研究。基于叶绿体基因组全序列，该研究对这 5 个淫羊藿物种进行了系统发育重建，结果支持淫羊藿与地理分布密切相关的属下大类群的划分，但也指出中国淫羊藿属植物的演化关系和属下系统划分还需要进一步的研究和完善。

原文链接：

The Complete Chloroplast Genome Sequences of Five Epimedium Species: Lights into Phylogenetic and Taxonomic Analyses

原文摘要：

Epimedium L. is a phylogenetically and economically important genus in the family Berberidaceae. We here sequenced the complete chloroplast (cp) genomes of four Epimediumspecies using Illumina sequencing technology via a combination of de novo and reference-guided assembly, which was also the first comprehensive cp genome analysis on Epimediumcombining the cp genome sequence of E. koreanum previously reported. The five Epimediumcp genomes exhibited typical quadripartite and circular structure that was rather conserved in genomic structure and the synteny of gene order. However, these cp genomes presented obvious variations at the boundaries of the four regions because of the expansion and contraction of the inverted repeat (IR) region and the single-copy (SC) boundary regions. The trnQ-UUGduplication occurred in the five Epimedium cp genomes, which was not found in the other basal eudicotyledons. The rapidly evolving cp genome regions were detected among the five cp genomes, as well as the difference of simple sequence repeats (SSR) and repeat sequence were identified. Phylogenetic relationships among the five Epimedium species based on their cp genomes showed accordance with the updated system of the genus on the whole, but reminded that the evolutionary relationships and the divisions of the genus need further investigation applying more evidences. The availability of these cp genomes provided valuable genetic information for accurately identifying species, taxonomy and phylogenetic resolution and evolution of Epimedium, and assist in exploration and utilization of Epimedium plants.

http://journal.frontiersin.org/article/10.3389/fpls.2016.00306/full

作者：王瑛