本研究以玉米优良自交系郑58的籽粒为材料,切取无菌苗的第一个茎节,上下各保留0.5 cm,接种于诱导培养基(1/2MS+ 0.3 mg/l NAA)培养,直接获得再生苗。腺苷二磷酸葡萄糖焦磷酸化酶(AGPase)是玉米淀粉合成过程中的一个限速酶。AGPase酶大亚基(AGPL)是酶的调节中心,在玉米胞质中过表达能够提高玉米淀粉含量。我们利用农杆菌介导法将玉米AGPL基因导入郑58的茎节体系,转化后的茎节在含有3 mg/l 双丙氨膦的1/2 MS培养基上进行筛选培养,对筛选后的抗性苗进行PCR和PCR-Southern blot检测,获得了4株转基因植株。
英文摘要:
The maize nodes were used as the explants to develop a regeneration system through organogenesis on the basal 1/2MS media added with 0.3mg/l NAA, and the upper and lower length of the node was 0.5 cm.ADP-glueose PyroPhosPhorylase (AGPase) is the key factor in the process ofSstarch synthase.The large subunit of AGPase gene (AGPL) is the active center,Over expression of which can increase the starch content.The ZmAGPL gene was transfered into maize nodes mediated by Agrobacterium tumefaciens, then the nodes were selected on the medium containing the concentration of bialaphos for 3mg/l. Four transgenic plants of ZmAGPL gene were gained which were confirmed by PCR and PCR-Southern.
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