Tree Genetic & Genomes:中科院武汉植物园韩月彭研究组发表苹果糖
近日，《Tree GENEtic & Genomes》上在线发表中科院武汉植物园韩月彭研究组发表关于控制苹果糖酸含量的遗传机制。马百全博士为论文第一作者，韩月彭研究员为论文通讯作者。
苹果中可溶性糖和有机酸的含量是决定果实口感品质的重要因素之一，武汉植物园果树分子育种学学科组马百全博士在韩月彭研究员的指导下，成功构建‘鸡冠’与‘望山红’杂交群体的高密度遗传连锁图，并对控制苹果中可溶性糖及有机酸含量QTL位点进行定位。研究发现：苹果第3号连锁群上存在一个控制苹果中可溶性糖组分(果糖、蔗糖、葡萄糖及山梨醇)含量的QTL簇(QTL Cluster)，并且在4号连锁群上存在着一个单独控制果实中葡萄糖含量的QTL位点。除此之外，在苹果8号与16号连锁群上分别存在着控制果实中苹果酸含量的QTL位点。该研究不仅增强了对苹果中控制果实口感品质的遗传机制的理解，而且为苹果中控制糖酸含量的分子标记辅助育种研究提供有意义的筛选标记。
苹果中控制可溶性糖及有机酸含量的QTL 定位
原文链接：
Construction of a high density linkage map and its application in the identification of QTLs for soluble sugar and organic acid components in apple
原文摘要：
Soluble sugars and organic acids have a strong impact on the overall organoleptic quality of fruits. In this study, we report the identification of quantitative trait loci (QTLs) for individual sugars and organic acids in apple. A high density linkage map of apple was constructed using the 1536 EST-derived SNP GoldenGate genotyping platform. The linkage map consists of 601 molecular markers, including 540 single nucleotide polymorphisms (SNPs) and 61 simple sequence repeats (SSRs), spanning 1368.4 cM with an average of 2.28 cM per marker. The contents of soluble sugars, including sucrose, glucose, fructose, sorbitol, and organic acids, including malic acid and citric acid, were used as the phenotypic data in QTL analysis. Two QTLs for malic acid content were detected on linkage groups (LGs) 8 and 16, while no QTL was found for citric acid content. Four QTLs for the glucose, sucrose, fructose, and sorbitol content were found to be clustered in one region on LG 3. Moreover, an additional QTL for glucose content was detected on the LG 4. Our study not only expands our understanding of the genetic basis for fruit organoleptic quality but it also provides molecular markers that will aid in marker-assisted selection for fruit quality in apple breeding programs.
作者：韩月彭
