J Exp Bot:中国农科院水稻所曾大力和钱前研究团队发表叶片早衰机
2015年12月25日，著名植物学期刊《Journal of Experimental Botany》在线发表中国农业科学院水稻研究所曾大力、钱前研究员带领的水稻功能基因组学创新团队的一篇研究论文，研究人员在叶片早衰机制研究上取得新进展。杨窑龙博士和徐杰博士为共同第一作者，曾大力研究员与钱前研究员为论文共同通讯作者。
研究人员借助图位克隆手段分离了水稻淡绿叶控制基因PGL，该基因编码水稻叶绿素a加氧酶，是叶绿素b形成过程中的关键基因，该基因的功能缺失会导致水稻叶片黄化、后期早衰加剧，进而影响水稻产量和品质。突变体pgl 还表现对高温的敏感，以及低光强下的活性氧清除系统受损，而致使水稻叶片表现早衰。利用该基因信息，通过对不同品种PGL等位基因变异的筛选和比较，将为探索抗早衰水稻新品种培育提供新思路。
叶片早衰是影响水稻产量和品质的重要因素。在水稻育种和栽培管理中，延缓叶片衰老成为保障水稻高产、稳产的重要手段。但长期以来，有关水稻叶片衰老的遗传调控机制仍不清楚。
原文链接：
PGL, encoding chlorophyllide a oxygenase 1, impacts leaf senescence and indirectly affects grain yield and quality in rICE
原文摘要：
Chlorophyll (Chl) b is a ubiquitous accessory pigment in land plants, green algae, and prochlorophytes. This pigment is synthesized from Chl a by chlorophyllide a oxygenase and plays a key role in adaptation to various environments. This study characterizes a rice mutant, pale green leaf (pgl), and isolates the gene PGL by using a map-based cloning approach. PGL, encoding chlorophyllide a oxygenase 1, is mainly expressed in the chlorenchyma and activated in the light-dependent Chl synthesis process. Compared with wild-type plants, pgl exhibits a lower Chl content with a reduced and disorderly thylakoid ultrastructure, which decreases the photosynthesis rate and results in reduced grain yield and quality. In addition, pgl exhibits premature senescence in both natural and dark-induced conditions and more severe Chl degradation and reactive oxygen species accumulation than does the wild-type. Moreover, pgl is sensitive to heat stress.
作者：曾大力和钱前
