玉米秸秆低温降解复合菌系GF-20的促分解作用及对土壤微生物多样性的影响
为评价复合菌系GF-20对玉米秸秆的促分解作用及对土壤微生物群落多样性的影响,在10℃黑暗条件下进行室内土培接种试验。在接种后15、30、45和60d分别测定GF-20及对照菌剂LK和SM的玉米秸秆降解率及土壤微生物群落结构。结果表明,与未施菌剂对照(CK)相比,施用菌剂在一定程度上加快玉米秸秆的分解,60d时菌剂处理较CK高出16.38%、6.60%和6.46%,以低温高效菌剂GF效果最佳,可明显加快秸秆分解进程。利用16SrDNA-PCR-DGGE技术检测土壤微生物群落的多样性,结果表明与未施菌剂对照(CK)和无秸秆无菌剂的空白对照(B)相比,施用菌剂对土壤细菌群落结构产生较大的影响,可显著提高土壤细菌富度(S)和多样性指数(H),表现为GF＞LK＞SM＞CK＞B；各处理之间的种群均匀度指数(E)差异不显著。玉米秸秆添加促腐解菌剂,可有效加快秸秆分解,增加土壤微生物群落多样性。
英文摘要:
In this study, to reveal the effects of microbial inoculants on corn stalk degradation and soil microbial diversity, corn stalk was used as organic material , three different microbial inoculants ( GF,LK and SM) were used in this study and cultured in the incubator under the dark culture condition at 10℃,an incubation experiment was carried out in the laboratory. Corn stalk degradation ratio and microbial biomass were measured at four different sampling times of 15d,30d,45d and 60d．The results showed that the decomposition of corn stalk could be enhanced by inoculation and degradation ratio of corn stalk in GF, LK and SM were 16.38%, 6.60% and 6.46% higher than that in CK, respectively. The 16SrDNA-PCR-DGGE fingerprinting profile of microbial indicated the composition and distribution of bands and cluster analyses based on the band similarity in migration and intensity between lanes were used to understand the distance of each lane. Results show that compared with CK and B, applying inoculants can significantly increase soil microbial Shannon diversity indices (H) and species richness (H), the species evenness index between the groups (E) no significant difference. In summary, corn stalk microbial inoculants could effectively accelerate corn stalk degradation and increase the soil microbial community diversity.
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