转异苞滨藜BADH基因玉米高世代株系苗期外源基因表达情况及耐盐性功能分析

发表日期：2015-12-30 12:04AM 阅览次数：

本研究以转来自耐盐植物异苞滨藜的甜菜碱醛脱氢酶(BADH)基因玉米T8代株系BZ-136及受体对照自交系郑58(耐盐)为试材,采用盆栽种植方法在三叶期时用250 mmol/L Nacl溶液持续浇灌9d,分析转基因植株中外源基因的表达情况,并检测耐盐相关生理指标,鉴定其耐盐性功能。结果表明,转基因株系BZ-136中的BADH酶活性及甜菜碱含量显著高于受体对照,并且随着盐胁迫时间的延长其表达量先增加后减少,在胁迫7d时分别达到了最大值；转基因株系幼苗株高和干重从盐胁迫第3d开始显著高于对照,鲜重和含水量分别在胁迫第5d和第7d时转基因株系显著高于对照；转基因株系根总体积显著高于对照,直径和根尖数在胁迫第5d时显著高于对照；转基因株系电导率和丙二醛含量均低于对照,分别在胁迫第7d和第3d开始差异达到显著水平,而叶绿素含量则高于受体对照,从第3d开始二者差异达到显著水平。可见由于外源BADH基因的表达转显著提高了基因株系的耐盐性。英文摘要: The T8 transgenic maize line BZ-136 with BADH gene from salt-tolerant plant Atriplexmicrantha was used in this study to analyze the exogenous gene expression and salt tolerance function under seedling stage.. The seedlings were planted in pot and were continuous treated by 250 mmol/L NaCl solutions from three-leaf period for 9 days. The results showed that the transgenic maize plants expressed higher amounts of betaine aldehyde dehydrogenase activity than the WT plants under NaCl stress and reached the peak till 7d. Growth of the transgenic maize plants was better than that of the WT plants under NaCl stress. Compared with the wild type, the transgenic plants had increased fresh weight, taller plant height, stronger root, lower malondialdehyde content, lower relative electrical conductivity, and higher chlorophyll content under salt stress, which indicated that the expression of BADH gene in maize seedlings enhanced the salt tolerance of these plants.

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