本研究以o2、sh2、su1、wx四个基因的近等基因系聚合材料为研究对象,用日立L-8900型全自动氨基酸分析仪对聚合材料籽粒中赖氨酸含量进行定量测定,以期了解o2与淀粉合成突变基因互作对籽粒赖氨酸含量的影响。结果表明,聚合材料赖氨酸含量介于0.35%-1.05%之间,郑58籽粒赖氨酸含量为0.28%,存在显著差异。o2与淀粉合成缺陷型突变基因(sh2、su1)互作可以显著增加赖氨酸含量,这三个基因聚合的三基因型o2sh2su1赖氨酸含量最高为1.05%,其次是sh2o2和su1o2,但是聚合材料粒重均不同程度下降；o2与淀粉修饰型突变基因(wx)互作赖氨酸含量未发生明显变化。su1o2以及su1o2wx既具有相对较高的赖氨酸含量又保持一定的粒重,这为高赖氨酸甜糯复合品质特用玉米新品种选育提供材料基础,为进一步探索基因互作增加赖氨酸的合成代谢途径及分子调控机制提供理论依据。
英文摘要:
The near-isogenic inbred lines of o2、sh2、su1、wx genes were studied to find the effect of o2 gene with starch-forming mutant genes on lysine content in maize kernel, and quantitative determination of lysine content was cayyied out by Hitachi L-8900 Amino-acid Analyzer.The results showed that the lysine contents in near-isogenic lines were 0.35-1.05%, while zheng58 inbred line was 0.28% and the difference was significant. The interaction of o2 with starch-deficient mutant genes(sh2,su1) results in increased amounts of lysine. Lysine content in the double mutant near-isogenic inbred lines(sh2o2, su1o2) was greater than in single mutant lines(sh2,su1,o2). The triple mutant sh2su1o2 was 1.05%, which was greatest, but the content of 100-kernel weight was reduced. A greater lysine content was not observed in the interaction of o2 with starch-modified mutant genes(wx).The su1o2 and su1o2wx had relative higher lysine content as well as a certain kernel weight, which provide material basis for further specialty corn breeding work and our study provide theoretical basis for exploration of the molecular mechanism of high lysine biosynthesis pathway.
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