PNAS:华东理工大学叶邦策教授发表放线菌调控氮代谢研究文章

摘要 : 12月7日，国际著名期刊《Proceedings of the National Academy of Sciences of the United States of America》在线发表华东理工大学叶邦策教授的研究论文

12月7日，国际著名期刊《Proceedings of the National Academy of sciences of the United States of America》在线发表华东理工大学叶邦策教授的研究论文，研究人员日前发现，氮代谢的核心调控子GlnR也控制着non-PTS(非磷酸转移酶系统)碳源的摄取和利用。叶邦策教授为论文通讯作者。

放线菌能够大量生产有益的代谢产物，在药物、化合物、能源等工业生产上有很大的价值。对多种碳源进行有效共利用，是放线菌工业生产的一个主要技术挑战。一直以来，人们对放线菌摄取和利用多种碳水化合物的调控机制知之甚少。

研究显示，放线菌GlnR不仅能够调控氮代谢，还控制着non-PTS碳源的ABC(atp-binding cassette)转运系统。GlnR可以根据细胞的营养状态，通过整合氮信号介导氮代谢和碳代谢的相互作用。这是首次在放线菌中系统描述GlnR介导的碳水化合物代谢调控。

原文链接：

Nitrogen regulator GlnR controls uptake and utilization of non-phosphotransferase-system carbon sources in actinomycetes

原文摘要：

The regulatory mechanisms underlying the uptake and utilization of multiple types of carbohydrates in actinomycetes remain poorly understood. In this study, we show that GlnR (central regulator of nitrogen metabolism) serves as a universal regulator of nitrogen metabolism and plays an important, previously unknown role in controlling the transport of non-phosphotransferase-system (PTS) carbon sources in actinomycetes. It was observed that GlnR can directly interact with the promoters of most (13 of 20) carbohydrate ATP-binding cassette (ABC) transporter loci and can activate the transcription of these genes in response to nitrogen availability in industrial, erythromycin-producing Saccharopolyspora erythraea. Deletion of the glnR gene resulted in severe growth retardation under the culture conditions used, with select ABC-transported carbohydrates (maltose, sorbitol, mannitol, cellobiose, trehalose, or mannose) used as the sole carbon source. Furthermore, we found that GlnR-mediated regulation of carbohydrate transport was highly conserved in actinomycetes. These results demonstrate that GlnR serves a role beyond nitrogen metabolism, mediating critical functions in carbon metabolism and crosstalk of nitrogen- and carbon-metabolism pathways in response to the nutritional states of cells. These findings provide insights into the molecular regulation of transport and metabolism of non-PTS carbohydrates and reveal potential applications for the cofermentation of biomass-derived sugars in the production of biofuels and bio-based chemicals.

doi: 10.1073/PNAS.1508465112

作者：叶邦策

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