Journal Proteomis:中科院武汉植物园杨平仿研究组发表莲愈伤蛋白质

摘要 : Journal of Proteomis在线发表中国科学院武汉植物园杨平仿研究组莲愈伤的研究结果，研究结果对于探讨莲愈伤组织形成的机理奠定基础。

Journal of Proteomis在线发表中国科学院武汉植物园杨平仿研究组莲愈伤的研究结果，研究结果对于探讨莲愈伤组织形成的机理奠定基础。水生植物基因组学与遗传育种学科组博士后刘艳丽为论文第一作者，杨平仿研究员为论文通讯作者。

莲是我国重要的水生经济作物，属于异花授粉植物。可以通过有性生殖(种子)和无性生殖(分藕)的方式产生后代。种子繁殖的方式产生的后代会发生性状分离，优良性状难以保持。生产中广泛应用的繁殖方式是分藕繁殖，但分藕繁殖成本高，且保存和运输费用高，繁殖系数低。

组织培养技术可在短时间内扩繁形成大量后代植株，但莲的组织培养鲜有成功报道。主要的障碍包括：难以产生愈伤;愈伤在继代过程中褐化严重难以保持;无形成完整植株的成功报道。因此深入开展莲的愈伤组织诱导工作，探讨影响愈伤组织形成的机理，为利用基因工程产生新资源奠定基础。

水生植物基因组学与遗传育种学科组博士后刘艳丽在杨平仿研究员的指导下，首先确立了白建莲的子叶胚的最佳愈伤诱导培养基，并利用蛋白质组学技术分析了子叶胚在未诱导、诱导10天和诱导20天三种条件下的蛋白质差异表达情况。结果表明子叶胚在不同诱导时间下参与代谢相关蛋白质、胁迫和氧化还原相关蛋白质、激素和信号传导相关蛋白质以及细胞壁相关蛋白质的表达水平发生显著变化。


图差异表达的蛋白质功能分类

原文链接：

Induction and quantitative proteomic analysis of cell dedifferentiation during callus formation of lotus (Nelumbo nucifera Gaertn.spp. baijianlian)

原文摘要：

Lotus is an aquatic plant with high nutritional, ornamental and medical values. Its callus formation is crucial for germplasm innovation by genetic transformation. In this study, embryogenic callus was successfully induced on appropriate medium using cotyledons at 12 days after pollination as explants. To dissect cellular dedifferentiation and callus formation processes at the proteome level, cotyledons before and tissues from 10 to 20 days after induction were sampled for shotgun proteomic analysis. By applying multivariate statistics 91 proteins were detected as differentially regulated, and sorted into 6 functional groups according to MapMan ontology analysis. Most of these proteins were implicated in various metabolisms, demonstrating that plant cells underwent metabolism reprogramming during callus induction. 14.3% proteins were associated with stress and redox, indicating that the detached explants were subjected to a variety of stresses; 13.2% were cell and cell wall-related proteins, suggesting that these proteins played important roles in rapid cell division and proliferation. Some proteins were further evaluated at the mRNA levels by quantitative reverse transcription PCR analysis. In conclusion, the results contributed to further deciphering of molecular processes of cellular dedifferentiation and callus formation, and provided a reference data set for the establishment of transgenic transformation in lotus.

doi:10.1016/j.jprot.2015.10.010

作者：杨平仿

;