以玉米高抗灰斑病自交系齐319与高感病自交系Ye478构建的RILS(重组自交系)为试材,通过两年田间表型鉴定,选取极端表型家系高抗16个,高感15个,利用SSR分子标记,并结合群体分离分析方法(BSA)筛选玉米抗灰斑病连锁标记并进行基因定位。结果表明,在玉米第1连锁群上检测到1个主效抗病基因位点(QTL),与两侧的分子标记umc2614和bnlg1803遗传图距分别为4.74cM和3.78cM,该抗病基因位点可解释40.9%的表型变异率,抗病基因来源于齐319,加性效应达到了-7.8175。这一研究结果为玉米抗灰斑病主效基因发掘和分子标记辅助育种奠定了基础。
英文摘要:
In this study, a maize RIL population derived from a cross between highly resistant Gray Leaf Spot inbred lines Qi319 and highly Susceptible Gray Leaf Spot inbred lines Ye478 was field-tested. The 16 highly resistant families and 15 highly Susceptible families were selected respectively to develop two DNA bulks based on the phenotypic identification results through artificial inoculation Gray Leaf Spot pathogen in two years. The bulks are screened for differences using simple sequence repeat(SSR) markers., and the markers were used for linkage analysis to locate the major QTL . The results show that one major Quantitative trait loci (QTL) was detected accurately on chromosome 1. The linkage distance to umc2614 and bnlg1803 are 4.74cM and 3.78cM respectively. The QTL explained 40.9% of the phenotypic variation,and additive genetic effects -7.8175. These results lay a foundation for GLS-resistance molecular marker-assisted selection (MAS) breeding and finding the major GLS-resistance gene .
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