导入碱蓬总DNA玉米后代耐盐生理指标测定及SSR标记分析
采用花粉管通道法，将野生碱蓬总DNA导入玉米自交系美系天杂。对导入后代M-3及受体进行0、150、200和250 mmol/L四个NaCl浓度梯度处理，结果表明：各处理浓度导入系干重、根和叶片中SOD和POD含量均高于受体且差异显著，MDA含量比受体低且差异显著。综合上述分析，推测导入系耐盐性有所提高。采用SSR标记进一步分析导入后代，引物phi065检测到导入系M-3与供体有相同带型。针对相同带型DNA片段测序分析，证明碱蓬DNA片段可能成功整合到玉米受体美系天杂基因组中，但整合碱基序列存在包括碱基的转换、颠换、缺失和插入四种类型变异。该实验再次证明了外源DNA导入方法是创造新种质的有效途径。
英文摘要:
The pollen tube pathway method was used to introduce suaeda salsa DNA into maize meixitianza inbred line. The offspring M-3 and receptor were treated by 0, 150, 200 and 250 mmol / L NaCl. The results showed that the dry weight, SOD and POD in the roots and leaves of M-3 were all higher than the receptor, and the content of MDA was lower under each treatment, significant differences. These results implied that the M-3 has a nice salt tolerance material. With the purpose of proof above results, the method of SSR marker analysis was used. The results showed that the same type line was detected by phi065 primers in the M-3 and Suaeda Salsa. Sequencing results indicated that the DNA fragment of Suaeda Salsa may successfully integrated into maize. But there were four types bases variation including transition, transversion, deletion and insertion in the integration fragment. This study demonstrated that introducing exogenous DNA into cultivated species is an effective way to create new germplasm resources.
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