Opaque2(O2)基因编码产生OPAQUE2蛋白,该蛋白为碱性亮氨酸拉链家族的一种转录因子,可作用于基因的启动区,调控基因的转录。辽2345/o2为本实验室构建的辽2345的opaque2(o2)基因回交导入系,本实验室在以前实验中的蛋白组学分析表明,辽2345与辽2345/o2在授粉后18天(days after pollination,DAP)的胚乳细胞中表达蛋白的种类和数量存在很大差异,并从中筛选出差异较明显的蛋白质9种。为了进一步验证O2与9种蛋白间的作用关系,本实验应用相对荧光定量PCR(realtime fluorescence quantitative PCR,qRT-PCR)的方法对9种蛋白进行了RNA水平表达量的检测。结果表明,在胚乳发育过程中Chfi很可能受到O2的抑制作用,而O2很可能对Ubc、Lgl、Sdh、LOC541920、Shmt、Ppdk等蛋白存在某种促进或激活作用。
英文摘要:
OPAQUE2, encoding by gene Opaque2,is one member of alkaline leucine zipper transcription factor family, and can regulate the expressions of several related genes by combining with the promoter region. Liao 2345/o2 is the gene backcross import department of o2 constructed by the laboratory. Obvious differences between protein expressions were verified by analyzing protein expressions in the endosperm of 18 days after pollination (DAP) using the method of two-dimensional electrophoresis, and 9 proteins were screened out through the mass spectrometry analysis. In order to determine the relationship between Opaque2 and the selected proteins, the amount of the RNA level expression of the selected proteins were detected using the relative fluorescence quantitative PCR (qRT-PCR). The result showed that Chif may be suppressed by O2, whereas Ubc, Lgl, Sdh, LOC541920, Shmt, and Ppdk were likely promoted or activated by O2.
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