解淀粉芽孢杆菌B9601-Y2对玉米的防病促生长效果研究*
为将解淀粉芽孢杆菌B9601-Y2(简称Y2)开发成防病促生微生物肥料，通过平板对峙培养及盆栽试验，研究了该菌株防治玉米小斑病和茎基腐病以及提高玉米生物量的效果。平板对峙培养结果表明，Y2发酵液、上清液及菌体悬液对玉米小斑病菌和茎基腐病原菌均有拮抗作用；盆栽实验结果显示，这3种处理对玉米小斑病防效60%左右，对茎腐病防效50%左右，防效依次为发酵液>菌体>无菌滤液。单独使用Y2发酵液、上清液及菌体悬液处理的苗根长、株高、鲜重和干重均显著高于清水和病原菌对照20%以上，它们喷施或灌溉接种病原菌的玉米苗生长量显著高于单独接种病原菌处理，有效地消除了病原菌对植株生长的影响。因此，生防菌Y2具有用于防治玉米病害和根肥及叶面肥的潜力。
英文摘要:
In order to develop Bacillus amyloliquefaciens B9601-Y2(Y2) into a biofertilizer with diseases control and growth promotion of maize, it was used to control southern leaf blight(SLB) and stalk rot and increase biomass of maize by dual-culture confrontation assay(DCA) and pot culture test. DCA showed that formulate suspension(FS), cell-free filtrate(CF) and cell suspension( CS) of Y2 could inhibit mycelial growth of Bipolaris maydis and Fusarium verticillioides. Pot culture test indicated that those suspensions could control over 60% of SLB and over 50% of stalk rot with the trendence FS > CS > CF.Those suspensions promoted over 20% of root length, plant type height, dry weight of above-ground and dry weight of under-ground for healthy maize and for the disease seedlings inoculated with B. maydis and F. verticillioides by spray or drenching. Therefore, Y2 has the potential serving as a root and leaf biofertilizer with disease control effect for maize.
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