Ac/Ds转座子系统及其在玉米突变体库构建中的应用
Ac/Ds转座子系统是研究玉米功能基因组学的重要工具。Ac/Ds转座子系统以其在基因组中的低拷贝、倾向插入基因外显子、可以在同一基因不同位点造成突变等独特优势, 在构建玉米突变体库和进行基因功能鉴定方面具有重要应用前景。综述了Ac/Ds转座子系统的插入特点、转座机制、在玉米突变体库构建中的应用以及我国玉米突变体库的现状, 并对我国玉米Ac/Ds突变体库的研究提出展望。
英文摘要:
Ac/Ds transposon system is an important tool for maize functional genomics. Its distinctive properties, e.g. low copy number in the genome, insertion basis to the exons, and mutating different loci in a single gene, make it a wonderful system for mutant library construction and gene function characterization in maize. The specificity and mechanisms of Ac/Ds transposon in maize as well as the application of this system in constructing the mutant library were reviewed. The current progress of mutant library construction and further utilization of Ac/Ds in China was also discussed.
董雷,库丽霞,陈彦惠,李平华.Ac/Ds转座子系统及其在玉米突变体库构建中的应用[J].玉米科学,2015,23(2):1~6
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