中科院海洋所绘制完成菊黄东方鲀基因组草图

菊黄东方鲀俗称“满天星”(图A)，隶属鲀形目、东方鲀科，在亚洲有着悠久的食用历史和极高的经济价值，也是我国重要的河鲀养殖品种之一。科研人员依托中科院海洋所自主构建的海洋生物基因资源平台和超算平台，独立完成了菊黄东方鲀(Takifugu flavidus)全基因组的测序、拼装及分析，利用SOLiD 4第二代高通量测序仪测序得到总长度76.0 Gb的序列，并创新性地采用“辅助拼接”策略拼接获得scaffolds 50,947条(scaffold N50为305,741 bp)。

研究发现，菊黄东方鲀基因组在GC含量和重复序列等多方面与红鳍东方鲀基因组相似，菊黄东方鲀基因组中共有30,285个蛋白编码基因，蛋白平均长度为519.9个氨基酸，获得51,990条GO注释信息(图B~D)，117条KEGG通路信息和270,803条InterPro注释信息。

该项研究积累了东方鲀属鱼类的遗传背景资料，为深度发掘海洋动物功能基因、开展蛋白功能的高效验证分析奠定了基础。相关研究结果在DNA Research发表。

原文摘要：

Draft Sequencing and Analysis of the Genome of Pufferfish Takifugu flavidus

Yang Gao, Qiang Gao, Huan Zhang, Lingling Wang,Fuchong Zhang, Chuanyan Yang and Linsheng Song

The pufferfish Takifugu flavidus is an important economic species due to its outstanding flavour and high market value. It has been regarded as an excellent model of genetic study for decades as well. In the present study, three mate-pair libraries of T. flavidus genome were sequenced by the SOLiD 4 next-generation sequencing platform, and the draft genome was constructed with the short reads using an assisted assembly strategy. The draft consists of 50,947 scaffolds with an N50 value of 305.7 kb, and the average GC content was 45.2%. The combined length of repetitive sequences was 26.5 Mb, which accounted for 6.87% of the genome, indicating that the compactness of T. flavidus genome was approximative with that of T. rubripes genome. A total of 1,253 non-coding RNA genes and 30,285 protein-encoding genes were assigned to the genome. There were 132,775 and 394 presumptive genes playing roles in the colour pattern variation, the relatively slow growth and the lipid metabolism, respectively. Among them, genes involved in the microtubule-dependent transport system, angiogenesis, decapentaplegic pathway and lipid mobilization were significantly expanded in the T. flavidus genome. This draft genome provides a valuable resource for understanding and improving both fundamental and applied research with pufferfish in the future.

作者：中科院