江苏农科院庄勇副组报道miRNA调控茄科抗盐胁迫

发表日期：2014-03-08 12:44PM 阅览次数：

茄科包括许多重要的蔬菜作物，它们经常遭受盐胁迫。microrna(miRNA)已被发现参与调控植物的基因表达来应答盐胁迫。然而，几乎没有关于茄科植物miRNA参与此类应答的报道。

由江苏省农业科学院蔬菜研究所庄勇副研究员领衔的课题组采用小RNA测序技术对此展开了深入分析，研究成果发表在近期的International Journal of molecular Sciences上。

为鉴定盐胁迫响应miRNAs，研究人员首先从NaCl处理和NaCl不处理的盐耐受品种—Solanum linnaeanum根中构建2个小RNA文库，并进行了高通量测序分析(由联川生物承担测序分析工作)。测序鉴定出来自37个家族的98个保守miRNAs，其中一些miRNA和其表达谱已被定量PCR验证。在盐胁迫下，11个miRNAs表达出现下调，3个miRNAs表达上调。

接着研究人员对这些盐胁迫应答miRNAs的靶基因进行了预测，发现它们参与了多种植物中的细胞进程。此项研究为miRNA在S. linnaeanum中的功能研究提供了有价值的信息，并有助于开发茄科作物的遗传改良策略。

原文摘要：

Conserved miRNAs and Their Response to Salt Stress in Wild Eggplant Solanum linnaeanum Roots

Yong Zhuang, Xiao-Hui Zhou and Jun Liu

The Solanaceae family includes some important vegetable crops, and they often suffer from salinity stress. Some miRNAs have been identified to regulate gene expression in plant response to salt stress; however, little is known about the involvement of miRNAs in Solanaceae species. To identify salt-responsive miRNAs, high-throughput sequencing was used to sequence libraries constructed from roots of the salt tolerant species, Solanum linnaeanum, treated with and without NaCl. The sequencing identified 98 conserved miRNAs corresponding to 37 families, and some of these miRNAs and their expression were verified by quantitative Real-Time PCR. Under the salt stress, 11 of the miRNAs were down-regulated, and 3 of the miRNAs were up-regulated. Potential targets of the salt-responsive miRNAs were predicted to be involved in diverse cellular processes in plants. This investigation provides valuable information for functional characterization of miRNAs in S. linnaeanum, and would be useful for developing strategies for the genetic improvement of the Solanaceae crops.

标签: 抗盐胁迫 miRNA 茄科

作者：联川生物点击：次

;