中科院版纳植物园完成蓖麻bZIP家族基因鉴定与表达分析

中国科学院西双版纳热带植物园分子遗传与育种组在蓖麻全基因组鉴定得到蓖麻bZIP家族所有成员，进一步对蓖麻bZIP家族基因结构、蛋白结构、二聚化特性、进化以及表达谱分析。相关文章发表于2014年2月份的《Planta》杂志上。



中科院版纳植物园完成蓖麻bZIP家族基因鉴定与表达分析

蓖麻是双子叶胚乳型植物的典型代表，是大戟科中重要的油料作物，其种子油被广泛地应用在工业上。bZIP(basic leucine zipper)转录因子广泛分布于真核生物中，在植物中涉及多种生物进程，其中ABF亚族更是ABA信号途径中重要的调控因子。

中国科学院西双版纳热带植物园分子遗传与育种组研究发现ABA对蓖麻油脂累积具有显著作用，bZIP转录因子可能参与ABA调控蓖麻油脂合成信号途径，为此研究人员在蓖麻全基因组鉴定得到蓖麻bZIP家族所有成员，进一步对蓖麻bZIP家族基因结构、蛋白结构、二聚化特性、进化以及表达谱分析。

得到以下结论：蓖麻bZIP家族成员49个，根据保守bZIP结构域特征将bZIP家族分为11个组(Group);对蓖麻bZIP转录因子结构域外的保守基序(Motif)、基因结构、进化关系等生物信息学分析，初步揭示了这两个家族成员在进化和功能上的相关性;对拟南芥和蓖麻中bZIP成员系统发生分析，均表明二者既存在同源性，又在进化中有基因丢失现象;基于DGE高通量表达测序数据以及公开的蓖麻五个组织RNA-Seq数据分别对蓖麻bZIP基因的组织表达模式分析，得到种子特异性表达的或者潜在与油脂相关的基因。

该研究结果为了解bZIP转录因子的结构和功能提供了重要信息。同时也为揭示蓖麻bZIP基因与蓖麻生长和发育，尤其是在种子储存物质累积中的作用提供了线索。

原文摘要：

Genomic surveys and expression analysis of bZIP gene family in castor bean (Ricinus communis L.)

Zhengwei Jin,Wei Xu, Aizhong Liu

The basic leucine zipper (bZIP) transcription factors comprise a family of transcriptional regulators present extensively in plants, involved in regulating diverse biological processes such as flower and vascular development, seed maturation, stress signaling and pathogen defense. Castor bean (Ricinus communis L. Euphorbiaceae) is one of the most important non-edible oilseed crops and its seed oil is broadly used for industrial applications. We performed a comprehensive genome-wide identification and analysis of the bZIP transcription factors that exist in the castor bean genome in this study. In total, 49 RcbZIP transcription factors were identified, characterized and categorized into 11 groups (I–XI) based on their gene structure, DNA-binding sites, conserved motifs, and phylogenetic relationships. The dimerization properties of 49 RcbZIP proteins were predicted on the basis of the characteristic features in the leucine zipper. Global expression profiles of 49 RcbZIPgenes among different tissues were examined using high-throughput sequencing of digital gene expression profiles, and resulted in diverse expression patterns that may provide basic information to further reveal the function of the 49 RcbZIP genes in castor bean. The results obtained from this study would provide valuable information in understanding the molecular basis of the RcbZIP transcription factor family and their potential function in regulating the growth and development, particularly in seed filling of castor bean.

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