本研究通过对大刍草转录组测序来发掘SSR标记位点。结果发现14 099个SSR位点,出现频率为 13.31%。三核苷酸和单核苷酸是主要重复类型,分别占SSR总数的42.12%和25.65%；六核苷酸重复所占比例最小,占0.43%。转录组结果表明,大刍草SSR中共发现38种重复基元,单核苷酸重复基元A/T出现频率最高,占总SSR的21.06%；其次为AG/CT和GCC/GGC,分别占 6.759%和 5.53%。5次重复的SSR数量最多,有3 927个,占总SSR的27.85%。本研究结果旨在为大刍草的SSR分子标记研究、遗传多样性分析、种群遗传结构等提供基础,以及大刍草基因发掘、分子标记辅助育种等工作。
英文摘要:
In this study, single sequence repeat(SSR)marker sites were identified based on transcriptome sequencing of teosinte(Z.mays. ssp. parviglumis).Results showed that a total of 14 099 SSR were obtained, and the SSRs occurrence frequency f was 13.31%.Trinucleotides and single nucleotides were the main types of repeats,accounting for 42.12% and 25.65% of the total SSRs, respectively;hexanucleotide repeats was the lowest which was 0.43%.Transcriptome results showed that a total of 38 repeat elements were found in the teosinte SSR, and single nucleotide repeat A / T exhibited the highest frequency,accounting for 21.058% of the total SSRs;followed by AG / CT and GCC / GGC,accounting for 6.76% and 5.53% respectively.The number of 5 repeat SSRs was the most,which was 3 927(27.85%).The results should contribute to researches
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