玉米大斑病菌对热带玉米种质CML493的DNA甲基化变异与mRNA表达分析

发表日期：2019-11-19 10:25PM 阅览次数：

以热带高抗大斑病玉米种质CML493幼苗为试验材料，采用人工接种大斑病菌接种处理，采用甲基化敏感扩增多态性(MSAP)技术和转录组测序(mRNA-seq)技术对经过接种大斑病菌处理不同时间的CML493基因组DNA胞嘧啶甲基化进行DNA甲基化模式、DNA甲基化水平分析和mRNA表达分析。结果表明：接种大斑病菌6d和12d的甲基化敏感扩增多态性分别为78.21%和76.13%，甲基化敏感扩增单态性分别为21.79%和23.87%。接种大斑病菌6d时，总甲基化率上升5.49%，全甲基化率上升14.24%，半甲基化率上升6.11%。接种大斑病菌12d时，总甲基化率上升6.40%，全甲基化率上升16.73%，半甲基化率上升5.57%。接种大斑病菌0d与6d和12d共检测到2298个共表达基因，共检测到1434个共表达上调基因，检测到769个共表达下调基因，上调基因数目显著多于下调基因数目。本研究共筛选出5个基因ID可能参与抗大斑病功能等调控，DNA甲基化变异可能与基因差异表达密切相关，其机理有待进一步挖掘。英文摘要: Tropical maize germplasm CML493 were artificial inocuting setosphaeria turcica in this study. The leaves surface present bit spot then gradually increase and enlargement along the extension of time. Methylation-sensitive amplification polymorphism (MSAP) technique and transcriptome sequencing (mRNA -seq) technique were used to analyze the DNA methylation pattern, DNA methylation level and mRNA expression of CML493. The results showed that the methylation sensitive amplification polymorphisms of each treatment group were 78.21% and 76.13%, and the methylation sensitive amplification polymorphisms of each treatment group were 21.79% and 23.87%. The total methylation rate increased by 5.49%, the total methylation rate increased by 14.24%, and the semi-methylation rate increased by 6.11%. When inoculated with bacteria solution after 6 days, the total methylation rate increased by 6.40%, the total methylation rate increased by 16.73%, and the semi-methylation rate increased by 5.57%. When inoculated with the bacteria solution on 12d, A total of 2298 coexpressed genes, 1434 coexpressed up-regulated genes and 769 coexpressed down-regulated genes were detected. The number of upregulated genes was significantly higher than that of down-regulated genes according to the volcanogram. A total of 5 gene IDs were screened out to be possibly involved in the regulation of inoculated Setosphaeria turcica. The total methylation and total methylation were closely related to the number of differentially expressed genes and down-regulated genes with the extension of the infection time.The number of hemimethylation was closely forecast related to the number of up-regulated genes .

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