异源表达玉米ZmWRKY11基因增强拟南芥对盐胁迫的耐受性
WRKY蛋白参与调控植物的生长发育及胁迫应答过程。本研究从玉米中分离得到一个响应高盐胁迫的WRKY基因,将其命名为ZmWRKY11。该蛋白定位于细胞核内且在酵母中不具备转录激活活性。酵母双杂交结果表明ZmWRKY11和ZmCaM2可以发生相互作用。为研究ZmWRKY11基因的生物学功能,构建了过量表达载体pCAMBIA1301-ZmWRKY11,并采用花序侵染法转化拟南芥。扩繁后对T3代转基因植株进行了耐盐鉴定试验。结果表明,转基因株系的绿苗率在盐胁迫处理后显著高于野生型拟南芥。同时,野生型株系中丙二醛含量和相对电解质渗透率相较于转基因植株发生了更为显著的上升,但脯氨酸含量的上升幅度则小于转基因株系。以上结果表明ZmWRKY11在拟南芥中的异源过表达增强了转基因植物对盐胁迫的耐受性。
英文摘要:
WRKY proteins were reported to participate in regulating plant development and adapting to diverse environmental stresses. In this study, WRKY11 encoding a WRKY transcription factor was cloned from maize. ZmWRKY11 expression was induced by salt stress. It is a nuclear located protein with no transcriptional activation ability in yeast. The interaction between ZmWRKY11 and ZmCaM2 was proved using yeast two hybrid assay. To validate the function of ZmWRKY11, we constructed an overexpression vector pCAMBIA1301-ZmWRKY11 and transformed the vector into Arabidopsis. Heterologous overexpression of ZmWRKY11 in Arabidopsis significantly enhanced plant tolerance to salt stress, as determined by physiological indicator such as cotyledons greening rate, malondialdehyde (MDA) content, relative electrolyte leakage (REL) and proline content. These findings suggested that ZmWRKY11 may function as a positive factor in plant salt stress response.
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