玉米叶片表皮蜡质在防止水分流失和紫外线损伤、应对逆境胁迫等方面有重要作用。叶片表皮有无蜡质外在体现为叶片本身是否具有挂水能力,因此,叶片挂水能力成为判断叶片表皮是否具有蜡质的一种重要参考标准。为发掘玉米叶片表皮蜡质合成与调控相关基因,本研究以218份由温带、亚热带和热带自交系组成的关联群体为材料,在原阳对其三个生物学重复叶片表皮挂水能力进行调查,利用1.25 M覆盖玉米全基因组的SNPs进行Q,K和Q+K三种模型下的全基因组关联分析。结果表明Q模型较K模型和Q+K模型能更好的评价叶片表皮的挂水能力。Q模型下,共检测到88个覆盖玉米9条染色体的显著SNPs(P ≤ 2.04E-6)。88个SNPs分布于47个QTLs内,单个QTL可解释13.6-45.6%的叶片挂水能力表型变异,47个QTL内共有97个候选基因,其中77个具有功能注释。位于第2染色体上的转录因子NAC77(GRMZM2G018436)和第3条染色体上的亚油酸脂氧合酶(GRMZM2G156861)编码基因,是叶片表皮蜡质性状的重要候选基因。
英文摘要:
Epicuticular waxes play an important role in preventing water loss, ultraviolet damage and coping with various stress. Water-holding capacity is the performance of wax content in leaf epidermis and becomes an important measurement of wax content in leaf epidermis. To identify candidate genes controlling and regulating epicuticular wax biosynthesis, the association panel of 218 inbred lines composed with temperate, subtropical, and tropical materials, were used. Their water-holding capacity was surveyed with three replications in Yuanyang. A
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