玉米热激蛋白ZmHSP70-12基因的克隆及表达分析
热激蛋白70(heat shock protein 70,HSP70)是原核和真核细胞中普遍存在的一种高度保守的分子伴侣。本研究从玉米中克隆了一个HSP70家族成员。该基因cDNA序列全长为1992 bp,开放阅读框为2352 bp,编码663个氨基酸,蛋白质分子量约75.0 kD,蛋白结构预测及同源比对分析表明,该基因编码蛋白含ATPase位点和HSP70保守结构域,与拟南芥AtHSP70-12序列高度相似,遂命名为ZmHSP70-12。蛋白亚细胞定位显示,ZmHSP70-12蛋白在内质网中表达。实时荧光定量PCR分析表明,ZmHSP70-12对非生物胁迫高温、干旱均具有明显的应答反应,从而推测ZmHSP70-12是玉米中与胁迫逆境相关的基因。
英文摘要:
Heat shock protein 70 (HSP70s) are found extensively in prokaryotic and eukaryotic and one of the most conserved molecular chaperone. In this study, a member of the HSP70 family was isolated from maize. It’s full-length coding sequence was 1992bp, and it’s open reading frame is 2352bp. The encoded protein with molecular weight is about 75.0kD.Through protein structure prediction and homologous alignment analysis, the gene encodes a protein containing ATPase site and HSP70 conserved domain. It is highly similar to Arabidopsis AtHSP70-12 sequence, so it is named ZmHSP70-12. Subcellular localization analysis showed that the ZmHSP70-12 protein was expressed in the endoplasmic reticulum. Real-time quantitative PCR analysis revealed ZmHSP70-12 has obvious response to abiotic stress, such as high temperature and drought.Therefore, it was speculated that ZmHSP70-12 is a stress-related gene in maize stress.
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