生长素反应因子ZmARF1的表达分析及互作网络探究
生长素在植物发育中的各个阶段都起着重要作用,而生长素反应因子特异性的结合长素应答基因启动子部位调控基因的表达,在植物的生长发育中起至关重要的作用。为了进一步研究玉米ARF家族成员ZmARF1的时空表达和旱响应模式及其互作网络。本研究以抗旱型郑36和弱抗旱型B73为试验材料,克隆及测序分析表明该基因含有一个2034bp的开放阅读框,翻译677个氨基酸；蛋白分析表明该蛋白无跨膜域,属于高亲水性蛋白,亚细胞定位于细胞核；潜在磷酸化位点分析显示,ZmARF1蛋白含有潜在的丝氨酸、苏氨酸和酪氨酸磷酸化位点数目分别为39个、16个和6个。复合进化树、功能域和保守motif分析表明该基因蛋白与其他物种同源性较高,说明不同物种该基因在功能上较保守；ZmARF1属于组成型表达基因,受旱胁迫的正向诱导,且郑36基因表达量上升幅度大于B73；预测的互作功能蛋白主要通过调节激素应答基因的表达、参与激素介导的信号等途径,来调控植物对逆境的胁迫应答和生长发育等过程。以上结果为进一步研究ARF类基因在玉米逆境胁迫和生长发育中的作用提供参考,亦为挖掘抗旱相关分子资源,进而培育抗旱品种奠定基础。
英文摘要:
Auxin plays an important role in all stages of plant development, and auxin-responsive binding in the promoter of the auxin-responsive gene regulates gene expression, which is essential in plant growth and development. In order to further study the spatiotemporal expression and drought response pattern, drought response and interaction network of ZmARF1.In this study, drought-resistant Zheng 36 and weak drought-resistant B73 were used as experimental materials. Cloning and sequencing analysis indicated that the gene contained a 2034 bp open reading frame and translated 677 amino acids.Protein analysis showed that the protein has no transmembrane domain and belongs to a highly hydrophilic protein. The subcellular localization is located in the nucleus.Analysis of potential phosphorylation sites revealed that the ZmARF1 protein contains 39, 16, and 6 potential serine, threonine, and tyrosine phosphorylation sites, respectively.The composite phylogenetic tree, functional domain and conserved motif analysis indicated that the homologous gene was highly homologous to other species, indicating that the gene is functionally conserved in different species.ZmARF1 is a constitutively expressed gene, which is positively induced by drought stress, and the expression level of Zheng 36 gene is increased more than B73. The predicted interaction function proteins mainly regulate the stress response and growth and development of plants under stress by regulating hormone response gene expression and participating in hormone-mediated signaling. The above results provide a reference for further study of the role of ARF genes in maize stress and growth and development, and lay the foundation for excavating drought-related molecular resources and breeding drought-tolerant varieties.
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