以不同血缘及不同世代的遗传育种材料为母本，高频孤雌生殖诱导系辽诱1和CAU5等为父本杂交诱导产生的单倍体籽粒为实验材料，研究秋水仙素浓度为0.625mg/ml的条件下，用喷淋加倍设备对试验材料进行8h的喷淋。结果表明：郑单958的S2代的材料加倍率最高，在33.75%，排在第二位的是L3258×H71是NSS种质加如旅大红骨后又加入黄改种质S0代材料，其加倍率为13.02%，第三名的是NSS种质加入热带种质S1代材料，加倍率是10.34%，加倍率最低的是辽黄群体，加倍率仅为0.33%，其次是S121/PH4CV是NSS种质加入旅大红骨种质S1代材料，其加倍率为0.57%，再次是L106/PH4CV是NSS种质加入热带种质S1代材料，加倍率是0.72%。利用该喷淋设备加倍单倍体籽粒的方法简便易行，但要对加倍的设备进行改进，对过程进行标准化，才能得到稳定的加倍效果。
英文摘要:
Using different generations of genetic breeding materials with different genetic background as female parent and high frequency parthenogenetic induction line G5 as haploid induced by male parent hybridization as experimental materials, the spraying doubling equipment was used to spray the experimental materials for 8 hours under the condition of colchicine concentration of 0.625 mg/ml. The results showed that the material doubling rate of Zhengdan 958 in S2 generation was the highest, at 33.75%. The second place was L3258×H71 which was added to NSS germplasm such as Ludahonggu and then to yellow-modified S0 generation. The doubling rate was 13.02%. The third place was that NSS germplasm added to tropical germplasm S1 generation. The doubling rate was 10.34%. The lowest was Liaohuang population, the doubling rate was only 0.33%. Secondly, S121/PH4CV was the S1 generation of NSS germplasm added to Luda Honggu germplasm. The doubling rate was 0.57%. Secondly, L106×PH4CV was the S1 generation of NSS germplasm added to tropical germplasm, and the doubling rate was 0.72%. However, statistical analysis showed that the doubling rate and material was 0.72%. The method of doubling haploid grains with the spraying equipment is simple and easy, but the doubling equipment should be improved and the process standardized, so as to obtain stable doubling effect.
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