玉米生长调节因子(GRF)家族的全基因组鉴定及在非生物胁迫下的表达研究

发表日期：2019-05-29 09:24PM 阅览次数：

生长调节因子(GRF)在调节植物生长发育以及响应非生物胁迫扮演重要角色。本研究以拟南芥作为种子序列进行Blastp序列比对,共鉴定15个玉米ZmGRFs基因并对其理化性质、系统进化关系、基因结构、保守基序、染色体定位、蛋白二级结构、磷酸化位点预测、玉米各组织部位表达谱及在非生物胁迫下的表达量进行分析。结果表明,根据系统发育树分析表明玉米ZmGRFs基因与单子叶植物水稻亲缘关系较近；15个ZmGRFs基因分别定位在1、2、4、5、6、7、9和10号染色体上且各个基因之间高度同源；每个亚族之间基因结构及保守基序均相对保守；玉米ZmGRFs蛋白二级结构均以α-螺旋和无规卷曲为主；根据磷酸化位点预测分析表明除ZmGRF6和ZmGRF9不含酪氨酸外,其它ZmGRFs蛋白均含有潜在的丝氨酸、苏氨酸和酪氨酸位点且各蛋白之间的潜在的磷酸化位点数目差异较大；根据转录组数据分析ZmGRF4和ZmGRF13基因在NaHCO3上调表达,同时发现热、盐和干旱胁迫下ZmGRF4和ZmGRF13基因表达量较高；冷胁迫下ZmGRF1/3/4/15基因表达量较高；根据玉米不同组织部位表达谱分析显示ZmGRF4和ZmGRF13在玉米各个部位表达量均较高,以上结果为进一步明确玉米GRF基因参与调节植物生长发育和非生物胁迫响应提供潜在的理论依据。英文摘要: Growth regulating factor (GRF) plays an important role in regulating plant growth development and responding to abiotic stress. In this study, Arabidopsis was used as seed sequence for Blastp sequence alignment. A total of 15 ZmGRF genes were identified and their physical and chemical properties, phylogenetic relationships, gene structure, conservative motifs, chromosome localization, protein secondary structure, prediction of phosphorylation sites, the expression profiles of different tissues and under abiotic stress expression were analyzed. The results show that according to phylogenetic tree analysis the relationship between GRF gene of maize and rice of monocotyledon plant was close; 15 ZmGRF genes were located on chromosomes 1, 2, 4, 5, 6, 7, 9 and 10 respectively, which there are highly homologous; gene structure and conserved motif function of each subgroup are relatively conservative; secondary structure of ZmGRF protein was mainly alpha-helix and random curl in maize; according to the prediction analysis of phosphorylation sites, except ZmGRF6 and ZmGRF9 not contain tyrosine, other ZmGRF proteins contain potential serine, threonine and tyrosine sites, and the number of potential phosphorylation sites varied greatly among proteins; transcriptome data analyzed the up-regulated expression of ZmGRF4 and ZmGRF13 genes under NaHCO3 stress, meantime, ZmGRF4 and ZmGRF13 genes were found to have high expression levels under heat, salt and drought stress; ZmGRF1/3/4/15 gene expression was higher under cold stress; the expression profiles of different tissues were analyzed showed that ZmGRF4 and ZmGRF13 were highly expressed in various parts of maize, further clarification of the involvement of GRF gene in plant growth development and abiotic stress response provides a potential theoretical basis. 查看全文

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