低温干燥贮藏的包衣和非包衣玉米种子活力及生理差异性研究
以玉米品种NK618和济单7号的包衣和非包衣种子为试验材料，低温干燥贮藏16个月、28个月和40个月时分别测定种子的发芽、活力指标，并测定幼苗的SOD、POD、CAT、MDA、脯氨酸等含量，旨在探讨低温干燥贮藏条件下玉米种子的可种用时间，比较包衣和非包衣玉米种子间的贮藏差异性及生理变化。结果表明：低温干燥条件下玉米种子贮藏16个月，包衣种子的各项发芽及活力指标比新种子显著降低，非包衣则无显著性变化；贮藏28个月，NK618和济单7号非包衣种子的发芽率分别是91%和87%，均可作种用，包衣种子已无种用价值；贮藏40个月时包衣和非包衣种子均不可作种用，包衣种子比非包衣种子更易老化；幼苗的SOD、POD、CAT含量均随贮藏时间延长而降低，28个月开始两品种包衣和非包衣三种酶含量均比对照新种子含量降低，但差异显著性不同。MDA和脯氨酸含量随贮藏时间延长而增加，到40个月时两品种的包衣和非包衣种子萌发幼苗MDA含量均达最大值。
英文摘要:
Coating and non-coating seeds of two maize varieties, NK618 and Jidan 7 were selected as experimental materials, When stored after 16, 28 and 40 months under low temperature and low humidity environment, the germination and vigor of seeds were tested, at the same time, the contents of SOD, POD, CAT, MDA, proline, etc. were tested after seeds were germinated. The purpose is to study the tolerance time used as sowing seeds under low temperature and low humidity conditions of storing and the difference between the coating and non-coating seeds of storage and physiology change. The results showed that maize seeds stored after 16 months under low temperature and low humidity condition, the germination and vigor index of the coating seeds decreased significantly than the contrast seeds, but the showed no significant difference; when stored after 28 months , the germination rate of non-coating seeds of NK618andJidan7 were 91% and 87% respectively，and could be used for sowing, but the coating seeds had no sowing value; All seeds stored after 40 months had no seed value. Coating seeds were easily aged than non-coated seeds. The contents of SOD, POD, and CAT in germinating seedlings all decreased along with extend of storage time. At 28 months, the contents of three protective enzyme were lower than the contrast new seeds, but the difference significance were difference. The contents of MDA and proline increased along with extend of storage time. Germination and vitality index are more sensitive to aging than physiological index. At 40 months the contents of MDA of the seedlings from the coating and non-coating seeds of two maize varieties were the maximum.
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