为了探讨不同基因型玉米对不同形态锰胁迫的响应机制，研究了垦粘1号（KN1），双玉201（SY201），东青1号（DQ1），吉农大516（JN516）和先玉335（XY335），及锰离子和络合态锰在不同浓度0mmol/L（Mn1）,0.005mmol/L（CK）,5mmol/L（Mn2），10mmol/L（Mn3），20mmol/L（Mn4）条件下，玉米植株氮代谢对锰胁迫响应。结果表明：随着锰浓度的升高，根系硝态氮含量下降,品种DQ1下降幅度最小。品种KN1和SY201铵态氮含量随锰浓度增加呈现先降低后升高趋势。品种JN1和DQ1随着锰胁迫增强，根部铵态氮含量下降。MnCl2·4H2O处理的玉米幼苗根系铵态氮积累程度大于EDTA-Mn处理。锰胁迫显著抑制根系可溶性蛋白合成。随着锰浓度增大，根系可溶性蛋白含量逐渐降低。MnCl2·4H2O处理根部可溶性蛋白下降程度大于EDTA-Mn处理。品种JN516根部可溶性蛋白含量最高。品种SY201可溶性蛋白合成受抑制程度较大。锰胁迫影响根部氮素养分的有效分配，阻碍韧皮部运输而使得部分氮素于根部积累。锰胁迫条件下，DQ1为耐受品系，SY201为敏感品系；游离态锰离子对玉米幼苗氮素转化抑制程度大于络合态锰离子。
英文摘要:
Taking five kinds of maize genotypes by regulating manganese concentration and manganese salt type as research object, analyzed effects of manganese stress on different varieties of nitrogen transformation. In this experiment, five kinds of maize varieties were cultivated with Kenji 1 (KN1), Shuangyu 201 (SY201), Dongqing 1 (DQ1), Ji Nongda 516 (JN516) and Xianyu 335 (XY335). Set five manganese concentration gradient respectively: (Mn1), 0.005mmol/L (CK), 5mmol/L (Mn2), 10mmol/L (Mn3), 20mmol/L (Mn4).Two kinds of form: MnCl2.4H2O and EDTA-Mn. The main results are as follows: With the increasing of manganese concentration, the content of nitrate nitrogen in root decreased, except for J, but the difference was not significant. DQ1 decreased the smallest. The content of ammonium nitrogen in KN1 and SY201 increased first and then decreased with the increasing of manganese concentration. The contents of ammonium nitrogen in JN516 and DQ1 decreased with the increasing of Mn stress. MnCl2·4H2O treatment of maize seedling root ammonium accumulation was greater than EDTA-Mn treatment. Manganese stress significantly inhibited the synthesis of soluble protein. With the increasing of manganese concentration, the content of soluble protein in roots decreased gradually. MnCl2·4H2O treatment of root soluble protein decreased more than EDTA-Mn treatment. The soluble protein content of root in JN516 was the highest among the five cultivars. Manganese stress affects the efficient distribution of nitrogen nutrients in the roots, hindering the transport of phloem and the accumulation of some nitrogen roots. Under the conditions of manganese stress, DQ 1 was a tolerant strain, and SY 201 was a sensitive strain; The inhibiting degree of free manganese ion on nitrogen transformation of maize seedling is greater than that of complexate manganese ion.
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