矮化玉米株型紧凑，适合密植，增产潜力较大，是玉米理想株型的重要决定因素之一。玉米矮杆突变体A2由PH6WC经EMS诱变获得，本研究对该突变体进行了表型鉴定、遗传分析、GA3敏感性和转录组分析。结果表明A2较其野生型株高显著降低10.13%、节间数减少和节间长度缩短，从而导致植株矮化；A2突变表型受单隐性基因控制；A2对赤霉素敏感，外源GA3可恢复其表型；转录组分析发现共有74个差异表达基因(DEGs)，KEGG分析表明这些DEGs显著富集在类胡萝卜素合成通路、戊糖和葡萄糖酸盐相互转化等通路上。本研究为深入研究该突变体并克隆相关基因奠定了基础。
英文摘要:
With compact plant type, high plant population and great potentialities for yield increase, drawf is one of the most important determinant of maize ideal plant type, A2 was identified by EMS treatment. Phynotyping, genetic analysis, exogenous GA3 sensitivity and transcriptome analysis were performed in this study. The results shows that the plant height of A2 decreased significantly by 10.13% due to less internodes number and shorter internodes. The dwarf trait was controlled by a single recessive gene. A2 was sensitive to exogenous GA3 which could recover plant height phenotype of A2 mutant. A total of 74 differentially expressed genes(DEGs) were identified by transcriptome sequecing. KEGG analysis shows that 74 DEGs classified into 4 pathways, including carotenoid biosynthesis, pentose and glucuronate interconversions and glycerophospholipid metabolism, etc. This study laid a foundation for further research and candidate gene cloning of A2 mutant.
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