生长素响应及转运信号在玉米不同节位腋芽发育过程中的分布模式分析
玉米多穗影响玉米的产量和品质,受环境因素及基因型等诸多因素调控。生长素作为一种重要的植物发育调控因子,它的代谢和转运在植物的器官形成和分化中均发挥重要作用。本文中,借助DR5rev::mRFPer和pZmPIN1a::ZmPIN1a::YFP的转基因植株,通过分析红色荧光蛋白标记的生长素响应信号和黄色荧光蛋白标记的生长素转运蛋白PIN1a的分布模式,发现在植株发育早期,不同节位上的腋芽处于同一发育时期,都还未发生成花转变,生长素响应信号和生长素转运蛋白的分布模式是一致的,均均匀分布在整个腋芽原基中；随着植株的生长,不同节位上的腋芽发育开始出现不同步,生长素响应信号和生长素转运蛋白的分布模式产生明显差异,位置越靠上的腋芽生长素响应信号越强,极性转运越活跃。这些结果都暗示生长素局部合成和极性转运在玉米腋芽发育过程中发挥作用。本文的结果将为全面解析生长素在玉米多穗形成过程中的作用机制提供有用的信息。
英文摘要:
The yield and quality of maize were influenced by maize prolificacy, which is regulated by many factors such as environment and genotype. As an important plant growth regulator, auxin plays an important role in organ formation and differentiation. In this study, the transgenic plants with DR5rev::mRFPer and pZmPIN1a::ZmPIN1a::YFP were used to analyze the distribution pattern of the auxin response signal labeled by red fluorescent protein and the auxin transport protein PIN1a labeled by yellow fluorescent protein, respectively. It was found that axillary bud of different nodes were at the same stage of development in the early stage of plant development. Auxin response signal and auxin transport protein are evenly distributed throughout the axillary bud of primordia. With the growth of the plant, the development of axillary buds at different nodes began to be out of sync. The patterns of auxin distribution and poplar auxin transport were significantly different between axillary buds at different nodes, the upper axillary bud showed stronger auxin-responsive signals and polar auxin transport signals. These results suggest that auxin local synthesis and polar transport play a role in the development of axillary buds of maize. The results of this paper will provide useful information for the comprehensive analysis of the mechanism of auxin in the process of maize multi-ear formation.
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