以耐盐玉米自交系‘M-2导入系’为试验材料，采用224对引物组合利用cDNA-AFLP (complementary DNA-amplified fragment length polymorphism)技术分析了玉米在4个不同浓度的盐胁迫诱导下的基因表达差异，对其差异片段回收测序，成功回收测序其中的71条TDFs(transcript-derived fragments)，用NCBI BlastX对测序结果进行比对分析，49条TDFs与已知蛋白功能基因有较高的同源性，涉及光合作用、细胞信号转导、胁迫响应、细胞代谢等多种途径，此外还有20个假设蛋白、未知蛋白和2个没有匹配的基因片段。利用qRT-PCR对TDF98、TDF3进行表达特征分析，结果表明这2个TDFs代表的基因在抵御玉米盐胁迫过程中起到了不同程度的调控作用。玉米盐胁迫相关基因表达情况较为复杂，涉及多种途径，本研究结果可为探讨玉米盐胁迫的分子机制和相关基因的克隆提供理论依据。
英文摘要:
cDNA-AFLP approach with 224 primer pairs was employed to analyze genes differentially expressed in normal and four stress maize inbred lines offspring induced by introducing suaeda salsa DNA into the ‘M-2 maize’. TDFs were successfully sequenced.According to the NCBI BlastX the comparative analysis of the sequencing results, 49 TDFs were homologous to genes involved in photosynthesis, cell signal transduction, stress response, cell metabolism and so on. The remaining 2 TDFs had no signal, 20 TDFs highly homology genes were unknown protein. Two TDFs (TDF3, TDF98) were selected for further study by real-time polymerase chain reaction (RT-PCR) that the expression of the defense genes responsive to play varying degrees of regulation roles in adapting to salt stress environment for maize. Genes involved in salt stress response were identified and their expression patterns successfully determined. This study laid the theoretical basis for the analysis of molecular mechanism and cloning of genes related with salt stress in maize.
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