以365份来源广泛的玉米自交系为材料，采用自主开发的55K芯片，在海南三亚、河南新乡和北京顺义3个环境下对玉米自交系株高进行全基因组关联分析。结果表明，株高在3个环境下变异非常丰富，有从低纬度到高纬度逐渐增加的趋势，株高在3个环境下均达到极显著相关，方差分析表明，株高在基因型、环境、基因型与环境互作方面均达到极显著差异水平。全基因组关联分析表明，不同环境下均检测到与株高显著关联的位点，在1号、3号、5号、6号、7号和8号染色体上均发现了显著关联的SNP，新乡和顺义环境下，在bin5.05区段发现了显著关联的SNP，海南和顺义环境下，在bin6.05区段发现了显著关联的SNP。对关联分析显著的SNP位点比对到参考基因组上，在maizeGDB网站上得到22个最可能的候选基因，该研究为探索玉米株高遗传机制提供了重要参考。
英文摘要:
Genome-wide association were conducted using phenotypic data from three environments (Sanya, Hainan; Xinxiang, Henan; Shunyi, Beijing) on a panel of 365 inbred lines and a set of 55K single-nucleotide polymorphism (SNPs) generated by Chip. The results showed that plant height varies very abundantly in three environments, and tends to increase gradually from low latitude to high latitude. Plant height has extremely significant correlation in all three environments. Variance analysis showed that plant height has extremely significant differences in genotype, environment, genotype and environment interaction. Genome-wide association study showed that significant association sites with plant height were detected in different environments. Significant association SNPs were found on chromosomes 1, 3, 5, 6, 7 and 8. In Xinxiang and Shunyi environments, Significant association SNPs were found in the bin5.05 region. In Hainan and Shunyi environments, significant association SNPs were found in the bin6.05 region. By comparing the significant SNP loci in association analysis with the reference genome, 22 most likely candidate genes were obtained in the MaizeGDB database . Once these SNPs are validated, they will be useful for marker-assisted selection and for a better understanding of maize plant height.
;
