摘 要:WOX是存在于植物体中的一类特异性家族基因，其参与了植物各个阶段的生命活动，具有调节植物干细胞的分裂分化等功能。本研究利用生物信息学的方法鉴定了31个玉米的WOX家族基因，并对这31个玉米WOX家族基因进行分析。多序列对比结果表明，玉米WOX家族和拟南芥WOX成员均具有高度保守的HOX结构域组helix1-loop-helix2-turn-helix3结构；以拟南芥WOX家族成员作为参照，构建系统进化树，玉米WOX家族成员和拟南芥WOX家族成员共46个基因被分为3个进化支。通过进一步的内含子以及motif分析，结果显示各个进化支内部的内含子和motif相似度极高，而不同的进化支间有较大的差别。对玉米WOX家族基因的表达模式和功能做了初步分析，结果表明有6个WOX家族基因参与了玉米生长发育的调控。
英文摘要:
WOX is a family of specific transcription factor family in plants, which is involved in the life activities of plants at various stages and has the functions of regulating the division and differentiation balance of plant stem cells. In this study, the maize (Zea mays) genome data was used to search and identify the three databases (Phytozome, PlantTFDB and NCBI). The 31 families of WOX family genes were identified . Multiple sequence comparisons revealed that the WOX family members of maize had a highly conserved HOX domain. The phylogenetic tree was constructed using the WOX family members of Arabidopsis thaliana as a reference, which were divided into 3 groups. Intron and motif analysis showed that the introns and motifs within each subfamily had extremely high similarities, and there were great differences among different subfamily. Finally, the expression patterns and functions of the WOX family genes in maize were analyzed. Made a preliminary summary.
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