玉米光周期敏感性基因ZmDPS10-2启动子结合蛋白筛选研究
以光敏感型热带玉米自交系CML288为试验材料,构建酵母单杂交文库,以ZmDPS10-2的核心启动子(1148bp)为诱饵,采用酵母单杂交技术从酵母单杂交文库中筛选到了33个与目的基因相互作用的蛋白,并根据基因功能注释,从中选出了4个与光周期、逆境响应相关蛋白PAO4、RPL23A、bZIP60和BAG。并构建了pGADT7载体做了进一步回转验证,初步证实了它们与候选基因的互作关系。结合启动子功能元件的预测,推测ZmDPS10-2候选基因可能在植物开花、ER胁迫、热胁迫等逆境调控途径中有一定的功能作用。该研究结果对深入解析该基因的分子机制及玉米光周期敏感性的遗传机理奠定了基础,对钝化玉米的光周期敏感性,培育早熟、抗倒、抗病的玉米新品种提供理论依据。
英文摘要:
A maizelight-sensitive tropical maize inbred line CML288 was used as experimental material to construct yeast one-hybrid library. The core promoter of ZmDPS10-2 (1148bp)was used as a bait, and use yeast one-hybrid technology, we got 33 genes interacting with ZmDPS10-2’s promoter. According to gene annotation, we selected 4 genes related to photoperiod and stimulus response，including PAO4、RPL23A、bZIP60 and BAG. These four genes were constructed into pGADT7 to confirm their interaction. Their interaction with ZmDPS10-2’s promoter was preliminarily confirmed. By bioinformatics analysis, it is speculated that ZmDPS10-2 may play a certain role in regulating plant flowering, ER stress and heat stress response. These results of this study laid the foundation for further analysis of the molecular mechanism of ZmDPS10-2 and the genetic mechanism of photoperiod sensitivity in maize. It provided a theoretical basis for the photoperiod sensitivity of passivated maize and the cultivation of new maize varieties with early maturity, lodging resistance and disease resistance.
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