以21份骨干玉米自交系为研究材料,于播种后21d至26d进行高温处理。测定株高、茎粗、叶面积、地上鲜重、地上部干重、总干重、胚根长、地下部分干重、根冠比、SPAD值等10个性状的耐热系数,运用主成分分析、隶属函数法、聚类分析及灰色关联度相结合的方法,对玉米自交系的耐热性进行综合评价。结果表明,主成分分析将10个单项指标转换为3个相互独立的综合指标,代表了全部数据83.160%的信息量。通过隶属函数法技术综合耐热评价值(D),并对其进行聚类分析,将21份自交系按耐热性强弱划分高耐热型(Ⅰ)、中耐热型(Ⅱ)、弱耐热型(Ⅲ)和热敏感型(Ⅳ)4类,筛选出2份高耐热型自交系KW4M029和DM08。总干重可以作为玉米自交系苗期简单、直观的耐热性鉴定指标。
英文摘要:
The objective of this study was to evaluate the heat resistance of 21 inbred lines of maize, which was based on heat treatment 21d to 26d after sowing. The heat comprehensive evaluation of heat tolerance coefficient of the plant height, stem diameter, leaf area, shoot fresh weight, shoot dry weight, total dry weight, root length, root dry weight, root shoot ratio, SPAD value of each inbred line was evaluated principal components analysis, membership function method, hierarchical cluster and gray relational grade analysis. The result showed that 3 independent comprehensive components ware obtained from 10 single indexes by principal component analysis, which reflected 83.160% information of all traits information. The comprehensive heat tolerance values(D) were calculated from the membership function methods, and 21 inbred lines of maize were divided into four heat tolerance types by cluster analysis,2 materials were high heat tolerance type(class Ⅰ, KW4M029 and DM08), 8 materials were medium heat tolerance type(class Ⅱ), 6 materials were weak heat tolerance type(class Ⅲ), 5 materials were heat sensitive type(class Ⅳ). Total dry weight can be used as simple and intuitive indices of heat resistance of inbred lines of maize at seedling stage.
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