玉米转录因子Zmhdz6的表达模式分析及互作功能蛋白预测
HD-Zip-I转录因子通过与其他蛋白互作,参与激素介导的信号途径,从而调控植物对逆境的胁迫应答和生长发育等过程。本研究利用RT-PCR方法从玉米基因组中克隆到GRMZM2G056600(Zmhdz6)转录因子的cDNA序列长786bp,编码261个氨基酸,分子质量为28.46kD。二级结构显示,编码的蛋白主要以α–螺旋和不规则卷曲为主,亚细胞定位预测显示,Zmhdz6定位于细胞核。进化树和motifs分析,发现Zmhdz6基因属于HD-Zip-I家族成员且与高粱同源达到99%,单子叶植物亚群含有7个重要的motifs；Zmhdz6基因蛋白互作预测,发现互作的基因主要参与逆境胁迫、信号传导和植物生长发育过程。荧光定量结果显示Zmhdz6在雌穗和雄穗中高度表达；Nacl、PEG胁迫和外源ABA诱导时均上调表达,以上结果说明Zmhdz6可能参与玉米逆境胁迫的应答和信号传导路径。
英文摘要:
HD-Zip transcription factor participates in hormone-mediated signaling pathways by interacting with other proteins,thereby regulating plant stress response and development processes.The GRMZM2G056600 (Zmhdz6) transcription factor was cloned from the maize genome by RT-PCR.The full-length cDNA sequence is 786 bp and encodes 261 amino acids,and the predicted molecular weights is 28.46kD. The predicted secondary structure suggested that the main structure were alpha helix and random coil.The subcellular local-ization analysis showed the Zmhdz6 protein were located in the nucleus.Analysis of phylogenetic tree and motifs revealed that the Zmhdz6 gene belongs to the HD-Zip-I family and has 99% homology with sorghum, and the monocot subpopulation contains seven important motifs;Zmhdz6 gene protein interaction prediction, found that the interaction genes are mainly involved in stress, signal transduction and plant development.Fluorescence quantification results showed that Zmhdz6 was highly expressed in the ear and tasse;Up-regulation of Nacl, PEG stress and exogenous ABA induction, the above results indicate that Zmhdz6 may be involved in the response of maize stress and signal transduction pathway.
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