染色体片段导入系群体作为次级作图群体,其遗传背景上仅有一到两个染色体片段来源于供体亲本,是精细定位控制复杂数量性状位点的理想材料。本研究以掖478为遗传背景、齐319为供体亲本的染色体片段导入系CL103为父本,与背景亲本回交并自交构建含955个单株的F2分离群体,对控制玉米雄穗分枝数的主效QTL开展精细定位。结果表明,2017年北京昌平环境下,两亲本雄穗分枝差异数达极显著水平,CL103平均雄穗分枝数为24.30,掖478平均雄穗分枝数为18.20,F2群体平均雄穗分枝数为22.03。参考B73RefGen_v3,利用完备区间作图法将qTBN7精细定位在第7染色体物理位置110,088,645-110,160,101 bp区域内,LOD值为25.06,解释11.47%的表型变异,该区间包含14个候选基因,包括已报道的控制玉米雄穗分枝数的基因ramosa1(ra1)。本研究为挖掘控制玉米雄穗分枝数基因及遗传改良奠定基础。
英文摘要:
Chromosome Segment Substitution Lines serve as secondary mapping populaions,each line has only one or two chromosome segments in the genetic background as donor fragments,which is an ideal material for QTL fine mapping.In this research,a F2 segregating population containing 955 individuals was constructed based on the female parent Ye478,the male parent CL103 derived from the receptor parent Ye478 and the donor parent Qi319 to map tassel branch number traits QTL.The main results are as following,under the environment of Changping in 2017,the tassel branch number of two parents reached an extremely significant level,the average number of CL103 was 24.30, Ye478 was 18.20 and F2 population was 22.03.Reference B73RefGen_v3,using composite interval mapping procedure to finely map qTBN7 to the 110,088,645-110,160,101bp region of chromosome 7,which LOD value was 25.06,accounted for 11.47 percent of the phenotypic variance,this interval contains 14 candidate genes,including the reported gene ramosa1(ra1)for the regulation of the tassel branch number.This study lays the foundation for control of gene function mining of maize tassel branch number.
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