为明确玉米线虫矮化病病原侵染后,玉米叶片病理反应的细胞学特征,利用透射电镜技术对健康和感病玉米叶片细胞超微结构进行了观察。结果表明:与健康植株相比,玉米线虫矮化病病原与玉米植株之间的互作都可以导致寄主发生明显的细胞病理学变化。在受侵染的玉米叶肉细胞中叶绿体膨胀变形、数量相对减少,双膜结构破损,基粒片层结构膨胀、排列无序、甚至解体;线粒体肿胀、变形、内嵴模糊严重者内嵴消失并空泡化,细胞核形态变为不规则,核膜破裂,染色质分布不均匀,呈降解状态。
英文摘要:
Histology and ultrastructure of maize leaves infected by pathogens causing maize nematode stunt disease were analyzed by using transmission electron microscopes.The results showed that marked cytopathological alterations were observed in infected leaves while compared with that in healthy plants.The chloroplasts in diseased leaves were deformed, and the number of cholroplasts were reduced. The double membrane structure were broken. The thylakoid lamellar structure were disordered or disintegrated. The abnormal mitochondria expanded with indistinct cristae. In serious abnormal mitochondria, of cristae disappearance and vacuolation of mitochondria with a few remains found. The abnormal cell nucleuses were found and envelope of nucleus was split. The chromatin distributed asymmetrically and degraded.
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