以玉米杂交种四单19及其双亲444和Mo17为试材，在Na2CO3溶液（25 mmol/L）和NaCl溶液（100mmol/L）胁迫下，进行种子萌发和苗期胁迫试验，测定发芽率、相对电导率、脯氨酸含量、超氧化物歧化酶活性、丙二醛含量等生理生化指标，比较杂交种与双亲自交系之间的耐盐碱差异。结果表明：（1）Na2CO3对于质膜透性的伤害程度大于NaCl；（2）发芽率（GP）、相对电导率（RC）、超氧化物歧化酶活性（SOD）、丙二醛含量（MDA）、脯氨酸含量（Pro）等五个指标均可用于玉米耐盐碱筛选鉴定。（3）玉米杂交种四单19及其双亲自交系的耐盐碱性强弱依次为四单19＞444＞Mo17，杂交种的耐盐碱性优于自交系。研究结果将为揭示玉米耐盐碱生理机制奠定实验基础。
英文摘要:
Under alkali-stress conditions (Na2CO3 25 mmol/L) and salt-stress conditions (NaCl 100 mmol/), maize hybrid Sidan19 and their parents were cultivated by sand culture. Some physiological and biochemical indicies were tested and used to evaluate salt-alkali tolerances of hybrids and their parents, which included germination percentage, relative conductivity, SOD activity, MDA content and proline content, etc. The main results were summarized as follows: (1) the damage degree of Na2CO3 to membrane permeability was more than that of NaCl; (2) germination percentage (GP), relative conductivity (RC), superoxide dismutase activity (SOD), malondialdehyde (MDA) content and proline content (Pro) were the appropriate physiological and biochemical indices；(3)the tolerance of hybrids Sidan19 and their parents was followed by Sidan19＞444＞Mo17, salt tolerance of the hybrid was superior to inbred lines. The results will lay a foundation for revealing the physiological mechanism of maize salt-alkali tolerant.
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