以耐涝性不同的近等基因系Zz-R和Zz-S为试验材料，采用盆栽淹水试验，研究淹水胁迫下玉米耐涝与不耐涝近等基因系生理指标的差异。结果表明：淹水胁迫下，耐涝系Zz-R超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、抗坏血酸酶（APX）活性与对照相比有所增加，且Zz-R增加幅度大于Zz-S。随着淹水时间的延长，Zz-R与对照相比仍表现为增加趋势，而淹水胁迫8d时，Zz-S与对照相比有所下降。淹水胁迫下Zz-R和Zz-S的MDA含量均高于对照组，且Zz-S增加幅度大于Zz-R,淹水胁迫8d时迅速积累，比对照增加320.5%。淹水胁迫下Zz-R和Zz-S的脯氨酸含量也显著高于对照，且Zz-R增加幅度大于Zz-S，淹水胁迫8d时，Zz-S脯氨酸含量与对照相比显著降低27%。近等基因系间耐涝性的差异与其抵御活性氧自由基(ROS)损伤能力的强弱、渗透调节能力的强弱有直接关系。
英文摘要:
The near-isogenic line Zz-R and Zz-S with different waterlogging tolerance were used to study the effects of waterlogging on the physiological characteristics by pot waterlogging experiment. The results showed that under waterlogging stress, the superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX) activity of waterlogging tolerance line Zz-R increased compared with the control, and the increasing scale of Zz-R was higher than Zz-S. With the prolongation of the waterlogging time, Zz-R still showed an increasing trend compared with the control. While at waterlogging stress 8d, the antioxidant enzyme activities of Zz-S decreased compared with the control. The MDA content of Zz-R and Zz-S under waterlogging stress were higher than the control group, and the increasing scale of Zz-S was higher than Zz-R. At waterlogging stress 8d, the MDA of Zz-S increased to the highest level, which increased 320.5% compared with the control. The proline content of Zz-R and Zz-S under waterlogging stress were also significantly higher than the control, and the increasing scale of Zz-R was higher than Zz-S. At waterlogging stress 8d, the proline content of Zz-S significantly reduced by 27% compared with the control. The difference of waterlogging tolerance between near isogenic lines is directly related to their ability to the resistance to ROS damage and osmotic adjustment.
查看全文
;
