本研究以转Cry11Ac基因玉米及其非转基因对照为试验材料,利用大、中、小不同孔径的凋落物分解袋,研究黑土区转基因玉米外源基因表达Bt蛋白的降解规律及其与土壤动物的互作关系。研究结果表明,第二年春天大孔径凋落物分解袋内转基因玉米残体Bt蛋白含量为初始含量的25.98%,至8月份Bt蛋白只剩下初始含量的0.35%；孔径对转基因玉米及其对照玉米凋落物分解率和Bt蛋白降解率均有显著影响,表现为大孔径>中孔径>小孔径,说明大型和中型土壤动物都可以促进凋落物分解和Bt蛋白的降解；与对照玉米相比,转基因玉米凋落物分解率和凋落物内土壤动物群落群落结构参数都没有显著差异(p>0.05, t-test),说明导入Bt基因对玉米残体分解速率和土壤动物均无显著影响。
英文摘要:
In this study, hybrids genetically modified maize with the Cry1Ac gene and its corresponding near isoline were used as test materials, the degradation rule of Bt protein and its interaction with soil fauna were studied by using litter bags of different mesh-sizes (large, medium and small aperture). The results showed that the content of Bt protein was 25.98% of the original content in big litter bag in the next spring, and to only 0.35% of the original content of Bt protein in August. Mesh size had a significant effect on the litter decomposition and Bt protein degradation, the results showed that large mesh size > middle mesh size > small mesh size. It was indicated that the large and the middle sized soil fauna played an important role in litter decomposition and Bt protein degradation. Litter decomposition rate and soil fauna community composition, community structure parameters in the genetically modified maize litter were not significantly different from its corresponding near isoline (p > 0.05, t - test). It was indicated that the import of the Bt gene had no significant effect on the litter decomposition and soil fauna in the litter.
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