增加玉米籽粒赖氨酸含量,改良玉米蛋白品质,一直以来是玉米品质改良的重要研究方向之一。本研究以玉米o2、sh2、su1、wx四个品质基因聚合的单基因、双基因、三基因近等基因系为研究对象,对籽粒氨基酸组分与醇溶蛋白SDS-PAGE带谱进行检测分析,以期了解品质基因聚合对籽粒氨基酸组分以及醇溶蛋白合成的影响。结果表明,与郑58相比较su1,wx,su1wx氨基酸组分变化较小,o2、sh2单基因以及含有o2或者sh2的双基因、三基因近等基因系中氨基酸组分变化较大,o2sh2、o2su1、o2sh2su1赖氨酸含量较高。SDS-PAGE显示o2与sh2、su1聚合导致19kD和22kD α-醇溶蛋白合成受到显著抑制。对这些聚合近等基因系的进一步研究将有助于我们加深对赖氨酸代谢途径和醇溶蛋白合成调控网络的认识,为利用基因工程技术改良籽粒蛋白品质提供理论依据。
英文摘要:
Improving nutritional quality of maize proteins by enhancement of lysine content have been a major topic of research for many years.The single, double and triple mutant nearly-isogenic inbred lines of opaque2, shrunken2, sugary1, waxy genes were studied to find the effect of o2 gene with starch-forming mutant genes on the amino acid compositions and zein synthesis, and the determinations were carried out by Hitachi L-8900 Amino-acid Analyzer and SDS-PAGE.The results showed that the amino acid composition of su1, wx, su1wx had minor changes while great change happened in single mutant o2 and sh2 and double mutant or triple mutant containing o2 or sh2. The lysine contents of o2sh2, o2su1 and o2sh2su1 were high. SDS-PAGE showed that the combination of o2 with starch-deficient mutant genes(sh2, su1) almost completely prevented the synthesis of 19kD and 22kD α-zein. Further study can provide a deeper theoretical understanding into lysine metabolic pathway and zein regulation network and provide theoretical basis to improve the quantity of maize protein through genetic engineering technology.
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