玉米突变体库创制及雄性不育突变体鉴定

通过钴60辐射诱变的京科糯2000获得了一个糯玉米突变体库。从该突变体库中鉴定出了71个不同类型的雄性完全不育的突变体。通过候选基因分析和表型-突变基因共分离鉴定,从无花粉突变体中鉴定到一个ms8突变体;一个花药缺失突变体鉴定为一个silky1突变体。这两个突变体均表现出雄性完全不育,而营养生长和雌穗结实正常,适用于雄性核不育杂交制种技术的研究和应用。 英文摘要: A mutant library of wax corn Jingkenuo2000 were made by 60Co-γ radiation. Seventy-five male sterile mutants were screened from it. A ms8 mutant without pollen grain and a silky1 mutant lack of anther were verified with sequencing and co-segregation of phenotypes and mutant alleles. Both mutants showed complete male sterility while normal seed-setting and vegetable development, which could be applied in new technology of hybrid production based on nuclear male sterility. 查看全文
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