玉米(Zea mays)苗期抗旱至关重要,采用称重控水法进行旱胁迫处理,设对照和胁迫两个处理,在3叶期对24个玉米自交系叶片相对含水量(RWC)和游离脯氨酸(pro)等生理指标进行测定,通过相关分析探索玉米在旱胁迫及复水过程中抗旱机理及补偿效应机制。结果表明,与对照相比,旱胁迫下叶片相对含水量、叶绿素荧光参数(Fv/Fm)和叶绿素含量下降,游离脯氨酸(pro)、超氧化物歧化酶(SOD)和氧化物酶(POD)上升趋势,复水后不同基因型自交系各生理指标表现出不同程度的补偿效应；利用隶属函数法筛选出2(D17982)、5(郑H71)、6(PH4CV) 、8(昌7-2)和9(L269)等14份抗旱性较强的自交系,根据复水后各生理指标的平均隶属函数值大小将6(PH4CV)、10(HCL645)、2(D17982)、19(郑7314)、5(郑H71)和14(郑9712)等归为复水恢复能力强的自交系。
英文摘要:
Drought-stress resistance is crucial for Corn seedling,In order to study the drought resistance mechanism under drought stress sitution and compensation mechanism after water recovery for maize , 24 inbred lines of maize were treated under control and drought stress treatments in the 3 leaf stage by weighing, leaf relative water content (RWC), free proline (pro) and other physiological indexes were determined by correlation analysis. The results show that,, the relative water content of leaves (RWC), chlorophyll fluorescence parameter (Fv/Fm) and the content of chlorophyll were descend, free proline (pro), superoxide dismutase (SOD), superoxide dismutase (POD) increased compared with the control under drought stress. Different genotypes of inbred lines showed various physiological indicators compensation effect in different degree after rewatering. using the membership function to screen 2 (D17982), 5 (Zheng H71), 6 (PH4CV), 8 (Chang 7-2), 9 (L269) and other 14 inbred lines with strong drought resistance, according to the physiological after water refers to the average membership function of the subject the values of 6 (PH4CV), 10 (HCL645), 2 (D17982), 19 (Zheng 7314), 5 (Zheng H71), 14 (Zheng 9712) and others belong to the complex water recovery ability.
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