Plant Physiol:中科院华南植物园张新华博士发表植物环境互作信号
近日，国际植物学顶级期刊《plant Physiology》杂志上在线发表了澳大利亚州立大学Whelan James教授与De Clercq Inge博士后研究组的一篇研究论文，研究报道了植物-环境互作信号分子MYB29研究进展。华南植物园张新华博士为论文第一作者，De Clercq Inge博士后为论文通讯作者。
植物通过不同的相互作用的信号转导途径感知和整合来自环境的各种激素和信号分子。细胞核编码的线粒体交替氧化酶(Alterative oxidase1a，aox1a)作为一个模式系统已经被用于研究线粒体和细胞核之间的逆行或压力信号(Retrograde signaling)。
华南植物园张新华博士于2014-2015年在澳大利亚拉筹伯大学访问学者期间，和Ivanova Aneta博士后在Whelan James教授与De Clercq Inge博士后指导下，鉴定了具MYB结构域蛋白AtMYB29，作为交替氧化酶基因的负调节因子(Regulator of alterative oxidase1a 7, [rao7] mutant)。在抗霉素A(Antimycin A)诱导下，rao7/myb29突变体与野生型比较具有更高的AOX1a表达与蛋白水平;多种与线粒体压力有关的基因表现为增强的转录丰度，表明RAO7/MYB29具有负向调控线粒体压力响应的作用。激素应答标记基因的Meta-分析和下游转录因子网络的鉴定揭示了MYB29具有复杂的相互调控网络，包括乙烯、茉莉酸、水杨酸、活性氧信号等各种乙烯响应因子和WRKY转录因子。尽管rao7/myb29增强线粒体压力响应基因的诱导，但是，在中度光与干旱作用下，它的突变体表现比较敏感，这些结果揭示了线粒体逆行信号和葡萄糖苷合成调节之间的相互作用。这种共同的调节者可以解释为什么线粒体功能扰动导致转录响应与生物胁迫响应的重叠。
原文链接：
The Transcription Factor MYB29 Is a Regulator ofALTERNATIVE OXIDASE1a1
原文摘要：
Plants sense and integrate a variety of signals from the environment through different interacting signal transduction pathways that involve hormones and signaling molecules. Using ALTERNATIVE OXIDASE1a (AOX1a) gene expression as a model system of retrograde or stress signaling between mitochondria and the nucleus, MYB DOMAIN PROTEIN29 (MYB29) was identified as a negative regulator (regulator of alternative oxidase1a 7 [rao7] mutant) in a genetic screen of Arabidopsis (Arabidopsis thaliana). rao7/myb29 mutants have increased levels of AOX1a transcript and protein compared to wild type after induction with antimycin A. A variety of genes previously associated with the mitochondrial stress response also display enhanced transcript abundance, indicating that RAO7/MYB29 negatively regulates mitochondrial stress responses in general. Meta-analysis of hormone-responsive marker genes and identification of downstream transcription factor networks revealed that MYB29 functions in the complex interplay of ethylene, jasmonic acid, salicylic acid, and reactive oxygen species signaling by regulating the expression of various ETHYLENE RESPONSE FACTOR and WRKY transcription factors. Despite an enhanced induction of mitochondrial stress response genes, rao7/myb29 mutants displayed an increased sensitivity to combined moderate light and drought stress. These results uncover interactions between mitochondrial retrograde signaling and the regulation of glucosinolate biosynthesis, both regulated by RAO7/MYB29. This common regulator can explain why perturbation of the mitochondrial function leads to transcriptomic responses overlapping with responses to biotic stress.
作者：张新华
