我国北方潮土玉米不同生长时期古菌群落结构和丰度的变化
采用16S rRNA基因末端限制性片段长度多态性分析(Terminal restriction fragment length polymorphism, T-RFLP)和实时荧光定量PCR(Quantitative real-time PCR, qPCR)技术，研究了玉米不同生长时期土壤古菌群落组成和丰度的变化。结果表明：1. 不同生长时期，根际土和非根际土古菌优势种群无显著变化，仅在各自样品中所占比例不同，古菌群落组成无明显分离。2. 乳熟期和完熟期古菌Shannon指数显著高于拔节期和抽雄期(P0.05)。3. 硝态氮是影响土壤古菌群落结构的关键因子。4. 土壤古菌16S rRNA基因丰度随生长时期的推进呈现先升高后降低的趋势，乳熟期最高，拔节期最低，乳熟期显著高于拔节期和完熟期(P0.05)。同一生长时期根际土和非根际土间无显著差异(P>0.05)。
英文摘要:
The molecular biological methods (terminal restriction fragment length polymorphism(T-RFLP) and Quantitative real-time PCR(qPCR) technique) based on the 16S rRNA gene were used to determine the community and abundance change of archaea in maize soil in different growth stages. The results showed: 1、No significant changes were observed in the dominant populations of rhizosphere and non-rhizosphere in different growth stages, only with different proportion in respective samples. There was no significant separation of the archaeal community composition. 2、The Shannon indexes at milky stage and ripening stage were significantly higher than that of jointing stage and tassel stage(P0.05).
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