为提高玉米单倍体加倍效率,本研究以0.06%秋水仙素和2%二甲基亚砜为加倍药剂,比较了不同处理时间、不同根长的玉米单倍体幼芽切根处理与直接浸根的加倍效果。结果表明：处理时间12h-72h均能提高玉米单倍体加倍率,显著高于自然加倍；处理48h加倍率最高；不同根长的切根处理加倍率存在显著差异,根长5-10mm加倍率最高,根长大于21mm加倍率最低。切根处理48h,单倍体植株平均散粉率和结实率分别为51.39%和8.74%,极显著高于直接浸根散粉率(44.88%)和结实率(4.42%)。
英文摘要:
To investigate the doubling efficiency (DE) of maize haploid, the colchicines (0.06%) and DMSO (2.0%) were used on the germ of root and cutting root of haploid which induced from DY37×S2187, and compared the DE with different treatment time and root length, respectively. The result indicated that DE for natural doubling were significantly lower than DE with a treatment of cutting root, which has been treated on colchicines (0.06%) and DMSO (2.0%) with 12 - 72 hours. Furthermore, the treatment time of 48 hours showed the most efficient on haploid doubling, the average rate of loose powder and seed set were 51.39% and 8.74%, which were significantly higher than the average rate of loose powder (44.88%) and seed set (4.42%) for germ of root with colchicines (0.06%) and DMSO (2.0%). In additional, the results suggested that the DE with 5-10 mm root length was most efficient, and DE of the root length longer than 21 mm were lowest. Keys words: Maize; root cutting; Haploid; double
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