J Hematol Oncol:浙江大学郭国骥教授课题组发表单细胞技术综述
近日，国际血液肿瘤学术期刊《JouRNAl of Hematology & Oncology》上在线发表了浙江大学医学院干细胞与再生医学中心郭国骥课题组题为“Studying hematopoiesis using single-cell technologies”的综述。直博生叶昉与本科生黄文韬为论文第一作者。
该综述系统的阐述了近年单细胞组学技术的进展，包括基因组，转录组，蛋白组和表观组的单细胞分析方法，以及单细胞分选、移植、成像等技术应用。该文回顾并评论了单细胞技术给造血系统研究所带来的突破性发现，包括对造血干细胞异质性的鉴定，造血干细胞的谱系分化路径，命运决定与调控网络的新模型，以及造血干细胞的发育起源等。最后，该文展望了单细胞技术的未来发展机遇与挑战。该文通讯作者是郭国骥教授。
原文链接：
Studying hematopoiesis using single-Cell technologies
原文摘要：
Hematopoiesis is probably the best-understood stem cell differentiation system; hematopoietic stem cell (HSC) transplantation represents the most widely used regenerative therapy. The classical view of lineage hierarchy in hematopoiesis is built on cell type definition system by a group of cell surface markers. However, the traditional model is facing increasing challenges, as many classical cell types are proved to be heterogeneous. Recently, the developments of new technologies allow genome, transcriptome, proteome, and epigenome analysis at the single-cell level. For the first time, we can study hematopoietic system at single-cell resolution on a multi-omic scale. Here, we review recent technical advances in single-cell analysis technology, as well as their current applications. We will also discuss the impact of single-cell technologies on both basic research and clinical application in hematology.
作者：郭国骥
