基于两种荧光毛细管电泳平台筛选评估玉米新型SSR引物
本研究以93份代表自交系及27套包括杂交种及双亲在内的三联体材料为试材，分别用Qiagen、ABI3730两种DNA分析仪，从111对新型玉米SSR引物中筛选出多态性高、稳定性好、适于自动化分型的引物9对，确定了一套适合SSR引物评估筛选的方案，从而为今后引物设计至评估入选提供高效的筛选方案奠定基础。
英文摘要:
In this study using the 93 inbred lines and 27 sets of mother-father-child trios containing hybrid and parents as experimental material,separately using two kinds of DNA analyzer to screening primers about Qiagen and ABI3730, screening 9 pairs of primers with high polymorphism,good stability and suitable for automatic classification from 111 pairs of primers,determined a set of suitable for SSR primers screening evaluating scheme,thus in order to lay a foundation for provide high efficiency screening scheme about primers design ,evaluate and choose in future.
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